PMID: 386339Jul 1, 1979Paper

Ten-nanometer filaments and mitosis: maintenance of structural continuity in dividing endothelial cells

Proceedings of the National Academy of Sciences of the United States of America
S H Blose

Abstract

By indirect immunofluorescence the behavior of the 10-nm filaments was studied at various stages of mitosis in guinea pig vascular endothelial cells. Interphase cells contain a ring of 10-nm filaments that encircles the nucleus and is maintained in a plane parallel to the substrate. During prophase and metaphase the cells round up and the 10-nm filament ring becomes wavy though still a closed structure. As anaphase progresses the ring then elongates into a rectangle that contains the spindle apparatus and chromosomes. In late telophase, cytokinesis cleaves the 10-nm filaments into crescents at the site of the contractile ring. These crescents then close into rings in the daughter cells. If cytokinesis is inhibited with 5 microgram of cytochalasin B per ml, then cleavage of the 10-nm filaments is blocked and the daughter nuclei remain surrounded by the parent ring. At no point during mitosis does the array of 10-nm filaments undergo major disassembly. These results indicate that, in contrast to the other major cytoplasmic structures, ventral microfilament bundles and cytoplasmic microtubules, which disassemble and reassemble during mitosis, 10-nm filaments remain intact throughout this process. The possibility is discussed that ...Continue Reading

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Related Concepts

Blood Vessel
Cell Cycle
Cytochalasin B
Microtrabecular Lattice
Endothelium
Immunofluorescence Assay
Cavia porcellus
M Phase, Mitotic

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