Tenascin-C hexabrachion assembly is a sequential two-step process initiated by coiled-coil alpha-helices.

The Journal of Biological Chemistry
R A KammererJ Engel

Abstract

We have investigated the oligomerization process of tenascin-C using a variety of recombinant wild-type and mutant polypeptide chain fragments produced by heterologous gene expression in Escherichia coli. Biochemical and biophysical analyses of the structures and assemblies of these fragments indicated a sequential two-step oligomerization mechanism of tenascin-C involving the concerted interaction of two distinct domains and cysteines 64, 111, and 113. First, the sequence between alanine 114 and glutamine 139 initiates hexabrachion formation via a parallel three-stranded coiled coil. Subsequently, the tenascin assembly domain, which is unique to the tenascins, is responsible for the connection of two triplets to a hexamer. The oligomerization of the tenascin assembly domains by the three-stranded coiled coil increases their homophilic binding affinity and is an important prerequisite for tenascin-C hexamerization. Although formation of the characteristic hexabrachion structure involves the covalent linkage of the six subunits by cysteine residues, mutational analysis indicates that hexamer formation is not dependent on intermolecular disulfide bonds. Most interestingly, substitution of glutamate 130 within the coiled-coil doma...Continue Reading

References

Jan 25, 1975·Journal of Molecular Biology·R P BywaterP A Timmins
Oct 25, 1975·Journal of Molecular Biology·A D McLachlan, M Stewart
Oct 1, 1992·Genes & Development·Y SagaS Aizawa
Feb 1, 1991·International Journal of Biological Macromolecules·J F Conway, D A Parry
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F S JonesG M Edelman
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J SodekL Jurasek
Sep 20, 1984·Nature·H P Erickson, J L Inglesias
Feb 17, 1995·The Journal of Biological Chemistry·D FischerM Chiquet
Sep 29, 1995·Experientia·R Chiquet-Ehrismann
Aug 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·R Timpl, J C Brown
Sep 1, 1994·Nature·P B HarburyT Alber
Aug 1, 1995·Protein Science : a Publication of the Protein Society·D N Woolfson, T Alber
Oct 1, 1996·Trends in Biochemical Sciences·A Lupas
Dec 1, 1996·The Journal of Cell Biology·R BrandenbergerM Chiquet
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·R A Kammerer
Feb 23, 1996·Science·K J Lumb, P S Kim

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Citations

Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·F S Jones, P L Jones
Dec 3, 1999·Biochemical and Biophysical Research Communications·E Di StasioA Brancaccio
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Jürgen Engel
Jan 20, 2004·Journal of Molecular Biology·Sergei P Boudko, Jürgen Engel
Apr 5, 2000·Progress in Neurobiology·P Pesheva, R Probstmeier
Dec 5, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·P L Jones, F S Jones
Mar 14, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·A Joester, A Faissner
Oct 21, 2006·Cell Death and Differentiation·I WatermannH Wajant
Sep 22, 1999·European Journal of Biochemistry·C DelmotteA Delmas
Dec 30, 2009·The Journal of Biological Chemistry·Hilka RauertHarald Wajant
May 29, 2010·The Journal of Biological Chemistry·Sergei P BoudkoHans Peter Bächinger
May 13, 2011·The Journal of Biological Chemistry·Matthew J StroudChristoph Ballestrem
Jun 16, 2012·PloS One·Neelanjana GhoshJordi Bella
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Richard A KammererMichel O Steinmetz
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·R A KammererM O Steinmetz
Oct 17, 2008·Kidney International·John Keeling, Guillermo A Herrera
Dec 9, 2014·Cell Adhesion & Migration·Sean P Giblin, Kim S Midwood
Mar 21, 2015·Cell Adhesion & Migration·Ulrich ValcourtLaurent Bartholin
Jul 31, 2013·The International Journal of Biochemistry & Cell Biology·Thomas L VincentJosephine C Adams
Jul 28, 2009·Journal of Molecular Biology·Sergei P BoudkoHans Peter Bächinger
Aug 4, 2016·Scientific Reports·Sarah BianchiRichard A Kammerer
Mar 25, 2017·FEBS Letters·Sebastian BittnerMartin Ehrenschwender
Jul 24, 2007·International Journal of Oral and Maxillofacial Surgery·A J Lyons, J Jones
Dec 17, 2008·Journal of Molecular Biology·Ashlee JollymoreHongbin Li
Aug 6, 2004·Protein Science : a Publication of the Protein Society·Manuela BozziAndrea Brancaccio
Jul 8, 2003·The Journal of Pathology·Fred T Bosman, Ivan Stamenkovic
Nov 3, 2006·FEBS Letters·Claire LethiasJean-Yves Exposito

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