Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment

International Journal of Molecular Sciences
Luisa BrandtJanina Burk

Abstract

Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represented by supplementation with interleukin-1β and/or tumor necrosis factor-α or by co-culture with allogeneic peripheral blood leukocytes, on ASC functional properties was investigated. High cytokine concentrations increased ASC proliferation and osteogenic differentiation, but decreased chondrogenic differentiation and ASC viability in scaffold culture, as well as tendon scaffold repopulation, and strongly influenced musculoskeletal gene expression. Effects regarding the latter differed between the monolayer and scaffold cultures. Leukocytes rather decreased ASC proliferation, but had similar effects on viability and musculoskeletal gene expre...Continue Reading

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Citations

Jan 16, 2020·International Journal of Molecular Sciences·Olivera EvrovaJohanna Buschmann
Jul 14, 2020·Frontiers in Veterinary Science·Metka VogaGregor Majdic
Dec 10, 2019·Npj Regenerative Medicine·Mark F PittengerArnold I Caplan
Sep 19, 2020·Journal of Tissue Engineering·Adam J JanvierJames R Henstock
Oct 10, 2020·World Journal of Stem Cells·Aleksandra JaukovićDiana Bugarski
May 21, 2021·Frontiers in Cell and Developmental Biology·Marguerite MeeremansCatharina De Schauwer

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Methods Mentioned

BETA
density gradient centrifugation
Fluorescence
PCR

Software Mentioned

ImageJ
Mathematica
SPSS Statistics

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