Tensile properties of single desmin intermediate filaments

Biophysical Journal
Laurent KreplakUeli Aebi

Abstract

Within muscle fibers, desmin intermediate filaments (IFs) are major constituents of the extrasarcomeric cytoskeleton. However, their contribution to the mechanical properties of myocytes has remained elusive. We present an experimental approach to measure the extensibility and the tensile strength of in vitro reconstituted desmin IFs adsorbed to a solid support. The tip of an atomic force microscope (AFM) was used to push on single filaments perpendicular to the filament axis. The torque of the AFM cantilever was monitored during the pushing events to yield an estimate of the lateral force necessary to bend and stretch the filaments. Desmin IFs were stretched up to 3.4-fold with a maximum force of approximately 3.5 nN. Fully stretched filaments exhibited a much smaller diameter than did native IFs, i.e., approximately 3.5 nm compared to 12.6 nm, both by AFM and electron microscopy. Moreover, we combined the morphological and lateral force data to compute an average stress-strain curve for a single desmin filament. The main features were a pronounced strain-hardening regime above 50% extension and a tensile strength of at least 240 MPa. Because of these nonlinear tensile properties, desmin IFs may dissipate mechanical energy and...Continue Reading

References

Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y TsudaT Yanagida
Mar 1, 1997·Biophysical Journal·M R FalvoR M Taylor
Feb 22, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sergei V StrelkovUeli Aebi
Jan 20, 2004·Journal of Molecular Biology·N MückeJ Langowski
Apr 28, 2004·Biophysical Journal·Sameer B ShahRichard L Lieber
Nov 13, 2004·The New England Journal of Medicine·M Bishr OmaryW H Irwin McLean
Oct 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Harald BärHarald Herrmann
Nov 1, 2005·Journal of Molecular Biology·L KreplakU Aebi
Jun 15, 2006·Nano Letters·Andreas HeidelbergJohn J Boland
Jul 11, 2006·Journal of Molecular Biology·Harald BärHarald Herrmann
Dec 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Laurent Kreplak, Douglas Fudge
May 19, 2007·Experimental Cell Research·Yassemi CapetanakiStelios Psarras
Jun 7, 2007·Nature Reviews. Molecular Cell Biology·Harald HerrmannUeli Aebi

❮ Previous
Next ❯

Citations

Jul 3, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Karl J A McCullaghKay E Davies
Nov 13, 2012·Acta Neuropathologica·Christoph S ClemenRolf Schröder
Dec 12, 2012·Cell and Tissue Research·Nickolay V BukoreshtlievAndrew E Pelling
Sep 8, 2012·Journal of the American Chemical Society·Artem ZhmurovJohn W Weisel
Apr 26, 2011·Computer Methods in Biomechanics and Biomedical Engineering·Melis ArslanMarkus J Buehler
Feb 25, 2011·Molecular Biology of the Cell·Brian T HelfandRobert D Goldman
Jul 1, 2009·Integrative and Comparative Biology·Douglas S FudgeG H McKinley
Nov 9, 2012·Proceedings. Biological Sciences·Daniel A Greenberg, Douglas S Fudge
Jul 10, 2009·The Journal of Clinical Investigation·Harald HerrmannUeli Aebi
Jun 24, 2009·PloS One·Theodor AckbarowMarkus J Buehler
Oct 8, 2013·PloS One·Bertrand-David SegardSabrina Batonnet-Pichon
Aug 3, 2010·HFSP Journal·Markus J Buehler, Yu Ching Yung
Jan 15, 2014·Cardiovascular Research·Giulio AgnettiJennifer E Van Eyk
Jul 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Atsuko UchidaAnthony Brown
Sep 7, 2011·The Journal of Cell Biology·Reinhard WindofferRudolf E Leube
Nov 12, 2013·Cell Communication & Adhesion·Robert M Harmon, Kathleen J Green
Aug 2, 2015·FEBS Letters·Micha KornreichRoy Beck
Nov 29, 2011·Journal of Structural Biology·Robert D GoldmanEdward R Kuczmarski
Nov 17, 2011·Journal of Structural Biology·Einat GrossmanOhad Medalia
Nov 26, 2011·Journal of Structural Biology·Shirley A Müller, Andreas Engel
Oct 9, 2009·Journal of Biomechanics·Zhao QinLaurent Kreplak
Dec 27, 2008·Journal of Molecular Biology·Kfir Ben-HarushOhad Medalia
Jan 13, 2009·Journal of Molecular Biology·Laurent KreplakJim O Vigoreaux
Nov 26, 2008·Journal of Molecular Biology·L Kreplak, H Bär
Nov 1, 2011·Journal of Molecular Recognition : JMR·Balázs KissMiklós S Z Kellermayer
May 16, 2015·Biochimica Et Biophysica Acta·Johanna BlockSarah Köster
Sep 10, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Ruiguo YangNing Xi
Mar 24, 2011·Journal of Structural Biology·Marija PlodinecCora-Ann Schoenenberger
Apr 22, 2014·Journal of Structural Biology·Balázs Kiss, Miklós S Z Kellermayer
Jan 27, 2015·Current Opinion in Cell Biology·Sarah KösterHarald Herrmann
Mar 27, 2010·Biophysical Journal·Gretchen A MeyerRichard L Lieber
May 9, 2009·Journal of Molecular Biology·Markus MeierPeter Burkhard
Jun 12, 2013·Experimental Cell Research·Qi Wen, Paul A Janmey
Sep 27, 2012·Biophysical Journal·Rustem I LitvinovJohn W Weisel
Feb 7, 2014·Critical Reviews in Food Science and Nutrition·Nafiseh Soltanizadeh, Mahdi Kadivar
Sep 30, 2016·Journal of Molecular Recognition : JMR·Ketaki ApteManfred Radmacher
Jan 5, 2017·Cold Spring Harbor Perspectives in Biology·Jonathan C R JonesKathleen J Green
Mar 24, 2018·Physical Biology·Yuval MullaGijsje H Koenderink
Aug 24, 2010·Bioinspiration & Biomimetics·Douglas S FudgeJohn M Gosline
Mar 15, 2018·Scientific Reports·Andrew S QuigleySamuel P Veres

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Laurent Kreplak, Douglas S Fudge
Nature Reviews. Molecular Cell Biology
Harald HerrmannUeli Aebi
© 2021 Meta ULC. All rights reserved