Tensilin-like stiffening protein from Holothuria leucospilota does not induce the stiffest state of catch connective tissue

The Journal of Experimental Biology
Masaki TamoriT Motokawa

Abstract

The dermis of sea cucumbers is a catch connective tissue or mutable connective tissue that exhibits large changes in mechanical properties. A stiffening protein, tensilin, has been isolated from the sea cucumber Cucumaria frondosa. We purified a similar protein, H-tensilin, from Holothuria leucospilota, which belongs to a different family to C. frondosa. H-tensilin appeared as a single band with an apparent molecular mass of 34 kDa on SDS-PAGE. No sugar chain was detected. Tryptic fragments of the protein had homology to known tensilin. H-tensilin aggregated isolated collagen fibrils in vitro in a buffer containing 0.5 mol l(-1) NaCl with or without 10 mmol l(-1) Ca(2+). The activity of H-tensilin was quantitatively studied by dynamic mechanical tests on the isolated dermis. H-tensilin increased stiffness of the dermis in the soft state, induced by Ca(2+)-free artificial seawater, to a level comparable to that of the standard state, which was the state found in the dermis rested in artificial seawater with normal ionic condition. H-tensilin decreased the energy dissipation ratio of the soft dermis to a level comparable to that of the standard state. When H-tensilin was applied on the dermis in the standard state, it did not alt...Continue Reading

References

Jun 1, 1990·The Biochemical Journal·R A WilliamsonR B Freedman
Jan 1, 1987·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·T Motokawa
Jan 1, 1981·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·T Motokawa
Aug 1, 1996·The Biological Bulletin·R Birenheide, T Motokawa
Jul 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·J A TrotterM A Atkinson
Jul 17, 1998·The Biological Bulletin·R BirenheideK Nomoto

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Citations

Nov 17, 2011·Cell and Tissue Research·Benjamin CharvetDominique Le Guellec
Mar 13, 2015·Journal of Biological Physics·Maya MizunoHiroaki Kojima
Sep 7, 2013·Marine Environmental Research·Michela SugniMaria Daniela Candia Carnevali
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jingyi MoHimadri S Gupta
Apr 13, 2018·PloS One·Igor Yu DolmatovAlexey V Boyko
Apr 5, 2017·The Journal of Experimental Biology·Mélanie DemeuldrePatrick Flammang
Oct 12, 2019·Biomimetics·Yusuke KobayashiTetsu Mitsumata
Jul 18, 2017·Journal of Anatomy·Liisa M BlowesMaurice R Elphick
Apr 10, 2021·Biomacromolecules·Dario FassiniRui L Reis
Oct 5, 2010·The Journal of Experimental Biology·Akira YamadaTatsuo Motokawa
May 18, 2010·The Journal of Experimental Biology·Masaki TamoriTatsuo Motokawa

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