Tension monitoring during epithelial-to-mesenchymal transition links the switch of phenotype to expression of moesin and cadherins in NMuMG cells

PloS One
David SchneiderAndreas Janshoff

Abstract

Structural alterations during epithelial-to-mesenchymal transition (EMT) pose a substantial challenge to the mechanical response of cells and are supposed to be key parameters for an increased malignancy during metastasis. Herein, we report that during EMT, apical tension of the epithelial cell line NMuMG is controlled by cell-cell contacts and the architecture of the underlying actin structures reflecting the mechanistic interplay between cellular structure and mechanics. Using force spectroscopy we find that tension in NMuMG cells slightly increases 24 h after EMT induction, whereas upon reaching the final mesenchymal-like state characterized by a complete loss of intercellular junctions and a concerted down-regulation of the adherens junction protein E-cadherin, the overall tension becomes similar to that of solitary adherent cells and fibroblasts. Interestingly, the contribution of the actin cytoskeleton on apical tension increases significantly upon EMT induction, most likely due to the formation of stable and highly contractile stress fibers which dominate the elastic properties of the cells after the transition. The structural alterations lead to the formation of single, highly motile cells rendering apical tension a goo...Continue Reading

References

Jun 1, 1976·Biophysical Journal·E A EvansL Melnik
Apr 1, 1996·The Journal of Cell Biology·R RamanathanM E Charness
Jan 1, 1996·Biophysical Journal·F M HochmuthM P Sheetz
Jul 1, 1997·The Journal of General Physiology·J DaiM P Sheetz
Dec 10, 1999·Biophysical Journal·J Dai, M P Sheetz
Jan 12, 2000·The Journal of Cell Biology·D Raucher, M P Sheetz
Feb 28, 2001·The Journal of Membrane Biology·C E Morris, U Homann
May 2, 2001·Nature Reviews. Molecular Cell Biology·M P Sheetz
Jul 20, 2001·Physical Review Letters·H U Voss
Oct 3, 2001·Physical Review Letters·B FabryJ J Fredberg
Nov 10, 2001·Annual Review of Biomedical Engineering·C ZhuN Wang
Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Jan 24, 2003·Annals of Biomedical Engineering·Warren D Marcus, Robert M Hochmuth
Mar 1, 2003·Biophysical Journal·Jordi AlcarazDaniel Navajas
Nov 19, 2003·Mechanisms of Development·David Shook, Ray Keller
Dec 20, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ben FabryJeffrey J Fredberg
Apr 16, 2004·Breast Cancer Research : BCR·Kimberly A BrownHarold L Moses
Jun 19, 2004·The Journal of Biological Chemistry·Mihály KovácsJames R Sellers
Sep 14, 2004·Biophysical Journal·E B LomakinaR E Waugh
Jun 7, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elizabeth D Hay
Dec 6, 2005·Biophysical Journal·Marco V BayasDeborah Leckband
Mar 29, 2006·The Journal of Cell Biology·Jonathan M LeeErik W Thompson
Aug 8, 2006·Biophysical Journal·Pere Roca-CusachsDaniel Navajas
Sep 30, 2006·Cell Motility and the Cytoskeleton·Hassina DarenfedCraig A Mandato
Apr 28, 2007·Annual Review of Biomedical Engineering·Paul A Janmey, Christopher A McCulloch
May 26, 2007·Science·Evan A Evans, David A Calderwood
Jun 7, 2007·Journal of Cell Science·Mingzhai SunGabor Forgacs
Jun 29, 2007·The Journal of Membrane Biology·Nicolas GroulxRyszard Grygorczyk
Mar 28, 2008·Nature Cell Biology·M KriegC-P Heisenberg
Feb 28, 2009·The International Journal of Developmental Biology·M Angela Nieto
May 22, 2009·The Journal of Biological Chemistry·Saumendra BajpaiDenis Wirtz
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Nov 13, 2009·Current Biology : CB·Ewa Paluch, Carl-Philipp Heisenberg
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Matthieu Cavey, Thomas Lecuit
Jan 30, 2010·Nature·Daniel A Fletcher, R Dyche Mullins
Feb 2, 2010·Nature Chemical Biology·Tilman Schneider-PoetschJun O Liu

❮ Previous
Next ❯

Citations

Aug 12, 2014·PloS One·Gautham VenugopalanChris H Rycroft
Oct 11, 2014·Experimental & Molecular Medicine·Ramadevi SubramaniRajkumar Lakshmanaswamy
Nov 7, 2015·Cytoskeleton·Sandeep M NalluriEsther W Gomez
Nov 4, 2015·Frontiers in Oncology·Vasiliki GkretsiTriantafyllos Stylianopoulos
Feb 26, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Thilo BaronskyAndreas Janshoff
Jul 21, 2015·Biochimica Et Biophysica Acta·Bastian R Brückner, Andreas Janshoff
Dec 3, 2015·Scientific Reports·Dongping QiAmy C Rowat
Apr 26, 2017·Nano Letters·Thilo BaronskyAlexey I Chizhik
Jan 9, 2015·Journal of the Royal Society, Interface·Jan RotherAndreas Janshoff
Aug 7, 2014·Clinical and Translational Medicine·Joseph W O'Connor, Esther W Gomez
Jan 2, 2019·International Journal of Molecular Sciences·Mariafrancesca CascioneGianluigi Giannelli
Jun 23, 2020·Biomicrofluidics·Molly Kozminsky, Lydia L Sohn
Jul 30, 2020·Scientific Reports·Nastasia V KoshelevaPeter S Timashev
May 11, 2018·Physical Biology·Ankur H KulkarniNamrata Gundiah
Mar 2, 2021·Frontiers in Cell and Developmental Biology·Galym IsmagulovGuojun Sheng
Jul 22, 2018·Progress in Biophysics and Molecular Biology·F Cavallini, M Tarantola
May 23, 2021·Biophysical Journal·Kamran HosseiniElisabeth Fischer-Friedrich
Jan 20, 2021·ACS Biomaterials Science & Engineering·Chong ShenQin Meng
Dec 3, 2021·Biochemical Society Transactions·Andreas Janshoff

❮ Previous
Next ❯

Methods Mentioned

BETA
FCS
fluorescence microscopy
AFM
rheology
force measurements
atomic force microscopy
Fluorescence

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.