Tension responses of chemically skinned fibre bundles of the guinea-pig taenia caeci under varied ionic environments

The Journal of Physiology
M Iino

Abstract

1. Thin fibre bundles (about 100 mum in width and 1.5 mm in length) were dissected from taenia caeci of the guinea-pig, and were mounted in an apparatus to record isometric tension mainly at 25 degrees C.2. Fibre bundles were chemically skinned by treating with 50 mug saponin/ml. for 20 min in a relaxing solution. Upon application of an activating solution containing 80 mum-Ca(2+) the fibre bundles rapidly developed a tension which was comparable in magnitude to that elicited with 10 mum-carbachol before the saponin treatment.3. It was confirmed that skinned fibre bundles develop active tension slowly in the virtual absence of Ca(2+) when Mg(2+) concentration was raised above 2 mm in the presence of MgATP. However, this tension was not developed in the presence of an ATP regenerating system.4. Maximum Ca(2+) activated tension and Ca sensitivity of skinned smooth muscle fibre bundles were examined using solutions in which pH, ionic strength, and the concentrations of Mg(2+) and MgATP(2-) were varied around supposedly physiological values. An ATP regenerating system was used when necessary.5. Maximum Ca(2+) activated tension changed only slightly with alteration in ionic strength (0.15-0.3 m). It decreased by 40% with increase in...Continue Reading

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