Teratocarcinomas Arising from Allogeneic Induced Pluripotent Stem Cell-Derived Cardiac Tissue Constructs Provoked Host Immune Rejection in Mice

Scientific Reports
Ai KawamuraYoshiki Sawa

Abstract

Transplantation of induced pluripotent stem cell-derived cardiac tissue constructs is a promising regenerative treatment for cardiac failure: however, its tumourigenic potential is concerning. We hypothesised that the tumourigenic potential may be eliminated by the host immune response after allogeneic cell transplantation. Scaffold-free iPSC-derived cardaic tissue sheets of C57BL/6 mouse origin were transplanted into the cardiac surface of syngeneic C57BL/6 mice and allogeneic BALB/c mice with or without tacrolimus injection. Syngeneic mice and tacrolimus-injected immunosuppressed allogeneic mice formed teratocarcinomas with identical phenotypes, characteristic, and time courses, as assessed by imaging tools including (18)F-fluorodeoxyglucose-positron emission tomography. In contrast, temporarily immunosuppressed allogeneic mice, following cessation of tacrolimus injection displayed diminished progression of the teratocarcinoma, accompanied by an accumulation of CD4/CD8-positive T cells, and finally achieved complete elimination of the teratocarcinoma. Our results indicated that malignant teratocarcinomas arising from induced pluripotent stem cell-derived cardiac tissue constructs provoked T cell-related host immune rejection ...Continue Reading

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Citations

Jul 30, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Alejandra Patino-GuerreroMehdi Nikkhah
Aug 10, 2017·International Journal of Molecular Sciences·Suet Lee Shirley DingPooi Ling Mok
Jan 6, 2018·Npj Regenerative Medicine·Luís Miguel MonteiroDiana Santos Nascimento
Oct 25, 2020·Stem Cell Reviews and Reports·Pallavi PushpMukesh Kumar Gupta
Jan 9, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Amy TurnerUlrich Broeckel

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