Teratoma Growth Retardation by HDACi Treatment of the Tumor Embryonal Source

Cancers
Jure KrasicNino Sincic

Abstract

Among testicular germ cell tumors, teratomas may often be very aggressive and therapy-resistant. Our aim was to investigate the impact of histone deacetylase inhibitors (HDACi) on the in vitro growth of experimental mouse teratoma by treating their embryonic source, the embryo-proper, composed only of the three germ layers. The growth of teratomas was measured for seven days, and histopathological analysis, IHC/morphometry quantification, gene enrichment analysis, and qPCR analysis on a selected panel of pluripotency and early differentiation genes followed. For the first time, within teratomas, we histopathologically assessed the undifferentiated component containing cancer stem cell-like cells (CSCLCs) and differentiated components containing numerous lymphocytes. Mitotic indices were higher than apoptotic indices in both components. Both HDACi treatments of the embryos-proper significantly reduced teratoma growth, although this could be related neither to apoptosis nor proliferation. Trichostatin A increased the amount of CSCLCs, and upregulated the mRNA expression of pluripotency/stemness genes as well as differentiation genes, e.g., T and Eomes. Valproate decreased the amount of CSCLCs, and downregulated the expressions of...Continue Reading

References

Jan 1, 1967·Advances in Morphogenesis·L C Stevens
Nov 19, 2003·Developmental Biology·David A F LoebelPatrick P L Tam
Nov 19, 2003·Biochemical and Biophysical Research Communications·Maksym KremenskoyKunio Shiota
May 20, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nadia GurvichJeremy B A Green
Jan 21, 2009·Reproduction, Fertility, and Development·Martha C GómezBetsy L Dresser
Aug 14, 2010·Toxicology and Applied Pharmacology·Emily W Y Tung, Louise M Winn
Mar 24, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Måns JergilMichael Stigson
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nicholas T CrumpLouis C Mahadevan
May 25, 2011·World Journal of Stem Cells·Naiara Z SaraivaJoaquim M Garcia
Feb 7, 2012·The American Journal of Pathology·Vivek VenkataramaniStefan Schweyer
May 3, 2012·Stem Cells International·Androniki KretsovaliNikolaos Charmpilas
Jul 5, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dafni MoschidouPascale V Guillot
Jan 18, 2013·PloS One·Colin T MaguireMaureen L Condic
Jun 21, 2013·The International Journal of Developmental Biology·Ximena Bustamante-MarínBlanche Capel
Dec 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Abdullah AldahmashAmer Mahmood
Apr 22, 2014·FEBS Letters·Anna ComelGiannino Del Sal
Dec 18, 2014·PloS One·Raymond M AnchanRichard L Maas
Oct 27, 2015·Nature Biotechnology·Alexander M TsankovAlexander Meissner
Nov 28, 2015·Surgical Pathology Clinics·Brooke E Howitt, Daniel M Berney
Dec 25, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Floriana Bulic-JakusNino Sincic
Aug 12, 2016·Reproduction, Fertility, and Development·Wannisa Sukhorum, Sitthichai Iamsaard
Aug 30, 2016·Human Pathology·Liang ChengLawrence M Roth
Oct 5, 2016·Journal of Cancer·Gabriela Figueroa-GonzálezCarlos Pérez-Plasencia
Jan 20, 2017·Experimental and Therapeutic Medicine·Zhijian SangYoucheng Yu
Feb 9, 2017·Scientific Reports·Megha Prakash BangaloreMitradas M Panicker
Apr 5, 2017·Stem Cells International·Gregory M Kelly, Mohamed I Gatie
May 6, 2017·Stem Cells International·Pedro M Aponte, Andrés Caicedo

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Methods Mentioned

BETA
histone acetylation
acetylation
Histone
electrophoresis
PCR

Software Mentioned

Image Lab
Search Tool for
Cytoscape
GraphPad Prism6

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis