PMID: 7031134Jan 1, 1981Paper

TerELISA: the ELISA test performed in Terasaki plates

Journal of Immunological Methods
E PaterakiS Avrameas

Abstract

The use of Terasaki (10 microliter samples) and microtitration (100 microliter samples) plates as the solid phase in enzyme immunoassays was compared. Various antigens were used for coating the plates and antibodies present in human sera were evaluated using the same anti-human Ig antibody labelled with either beta -galactosidase, alkaline phosphatase, peroxidase or glucose oxidase. The results obtained, either by scoring with the naked eye or by absorbance reading with appropriate densitometers, showed that both plates were equally suitable and that the 4 enzymes were equally effective in detecting the same lowest quantity of antibody. A comparative evaluation using either Terasaki or microtitration plates for the quantitation of human anti-Echinococcus granulosus antibody ii 50 sera demonstrated that there was a good correlation between the two procedures (r = 0.8097). Finally, the use of glucose oxidase as the enzyme marked allowed a clear-cut distinction to be made between positive and negative samples with the naked eye alone.

References

Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L GuesdonS Avrameas
Nov 1, 1971·European Journal of Immunology·S Avrameas, B Guilbert
Jun 24, 1971·FEBS Letters·B K. Van Weemen, A H.W.M. Schuurs

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Citations

Aug 1, 1989·Biulleten' eksperimental'noĭ biologii i meditsiny·G V Pinchuk, L N Pinchuk
Aug 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M G GabridgeM F Gladd
Feb 26, 1987·Journal of Immunological Methods·H Durbin, W F Bodmer
Apr 8, 1991·Journal of Immunological Methods·M C Cerrone, R E Kuhn
Mar 3, 2009·Colloids and Surfaces. B, Biointerfaces·Satoshi Sasaki, Yoshinori Iida
Mar 1, 1984·Immunology Today·J J HaaijmanJ Radl

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