Abstract
The membrane attack complex (MAC) of carp complement was extracted with deoxycholate from rabbit erythrocytes lysed by carp serum and purified by a two-step chromatographic procedure. On two-dimensional SDS-PAGE of carp MAC, eight bands were detected. The band of M(r) 91,000 was identified as carp C9 by western blotting using anti-carp C9, and two bands of M(r) 62,000 and one band of M(r) 22,000 were confirmed as those of carp C8 alpha, C8 beta and C8 gamma, respectively, by their N-terminal amino acid sequences. The bands of M(r) 102,000 and 73,000, which generated from a 180,000 band under reducing conditions, were those corresponding to human C5b alpha and C5b beta, respectively. The remaining bands of M(r) 115,000 and 106,000 were identified as those corresponding to human C6 and C7, as determined by their molecular size, single-chain structures and similarities in N-terminal amino acid sequences to their mammalian counterparts. Densitometric scan of the gels showed the molar ratio of C5b, C6, C7, C8 and C9 in carp MAC to be 1:1:1:1:4. Based on these results, it appears that, as with mammals, the cytolytic pathway of bony fish complement is composed of five terminal components from C5 to C9.
References
Sep 1, 1991·Immunology Today·T Kinoshita
Sep 1, 1991·Immunology Today·T C Farries, J P Atkinson
Jan 1, 1987·Developmental and Comparative Immunology·T L Koppenheffer
Feb 15, 1987·Experientia·C Canicatti, F Veuthey
Jan 1, 1985·Developmental and Comparative Immunology·C W Vogel, H J Müller-Eberhard
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1966·Analytical Biochemistry·C B Laurell
Jul 1, 1984·Molecular Immunology·E R Podack, J Tschopp
Jan 1, 1984·Springer Seminars in Immunopathology·H J Müller-Eberhard
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C F Ware, W P Kolb
Mar 1, 1983·The Biochemical Journal·S Bhakdi, J Tranum-Jensen
Oct 30, 1981·Science·J A JensenM Cayer
Sep 1, 1981·Analytical Biochemistry·S M Read, D H Northcote
Jan 1, 1993·Developmental and Comparative Immunology·S TomlinsonA F Esser
Aug 1, 1996·Molecular Immunology·T UemuraM Nakao
Citations
Jan 28, 1998·Molecular Reproduction and Development·P H Lee, F W Goetz
Apr 23, 2013·Developmental and Comparative Immunology·W D Niroshana WickramaarachchiJehee Lee
May 18, 2001·Developmental and Comparative Immunology·S FranchiniJ D Lambris
Oct 17, 2001·Developmental and Comparative Immunology·B G Magor, K E Magor
Oct 17, 2001·Developmental and Comparative Immunology·I K ZarkadisJ D Lambris
Jan 23, 1999·Immunological Reviews·M Nakao, T Yano
Jan 23, 1999·Immunological Reviews·J O Sunyer, J D Lambris
Jan 23, 1999·Immunological Reviews·S L Smith
Oct 13, 2012·Developmental and Comparative Immunology·W D Niroshana WickramaarachchiJehee Lee
Jun 10, 2005·Xenotransplantation·Debbie A PlouffeMiodrag Belosevic
Jul 17, 2012·Fish & Shellfish Immunology·Wickramaarachchige Don Niroshana WickramaarachchiJehee Lee
Aug 27, 2002·Fish & Shellfish Immunology·M Claire H Holland, John D Lambris
Jul 3, 2007·Veterinary Immunology and Immunopathology·L LiP Nie
Mar 24, 2016·International Journal of Molecular Sciences·Huan ZhengJonathan P A Gardner
Mar 6, 2003·Immunogenetics·Yoko KatoTomoki Yano
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miki NakaoTomoki Yano
May 29, 2007·Molecular Immunology·Guozhu LiuXinhua Chen