Terminal differentiation of T cells specific for mutant H-2K antigens. Conversion of Lyt-1,2 cell into Lyt-2 but not Lyt-1 cells, in vitro

European Journal of Immunology
Z A NagyJ Klein

Abstract

The Lyt phenotype of T cells at different stages of response to mutant H-2K antigens was determined by immunofluorescence using monoclonal rat anti-Lyt antibodies. Previous observations indicated a differential expression of the two allelic forms of Lyt-1 antigen on these cells. Since the rat antibodies recognize nonpolymorphic framework determinants of Lyt antigens, in our approach the expression of both Lyt-1 alleles was analyzed with the same antibody. It was found that cells reacting to three different H-2K mutants have the Lyt-1,2 phenotype, regardless of the Lyt-1 allele carried by the responder strain. The Lyt phenotype of responder cells remained unchanged after priming in vivo. However, cells recovered from cultures after secondary stimulation in vitro were mainly Lyt-2, with few Lyt-1,2 and virtually no Lyt-1 cells present. This change of Lyt phenotype ran in parallel with the loss of proliferative capacity to the priming antigen, but cytolytic activity of the cells remained unimpaired. Long-term proliferation of T cells induced against mutant H-2K antigens could only be maintained in the presence of a T cell growth factor. Cultures with growth factor contained almost exclusively Lyt-2 cells and exerted strong cytolyt...Continue Reading

References

Mar 1, 1976·The Journal of Experimental Medicine·Z NagyB Pernis
Oct 1, 1976·The Journal of Experimental Medicine·H ShikuH F Oettgen
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E NakayamaL J Old
Jan 1, 1979·Scandinavian Journal of Immunology·B J Alter, F H Bach
Sep 1, 1978·The Journal of Experimental Medicine·F H Bach, B J Alter
May 1, 1978·The Journal of Experimental Medicine·P J WettsteinJ A Frelinger
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P J WettsteinJ A Frelinger
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·H Cantor, E A Boyse
Jan 1, 1978·Advances in Immunology·J Klein
Jan 1, 1979·Immunological Reviews·J A Ledbetter, L A Herzenberg
Feb 1, 1980·The Journal of Experimental Medicine·C E Hayes, F H Bach

❮ Previous
Next ❯

Citations

Jan 1, 1982·Immunogenetics·C MarkJ Klein
Jun 1, 1986·Immunological Reviews·J SprentR Korngold
Jun 1, 1985·Cell Biophysics·T N Buican, G W Hoffmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Experimental Medicine
F H Bach, B J Alter
Proceedings of the National Academy of Sciences of the United States of America
P J WettsteinJ A Frelinger
© 2021 Meta ULC. All rights reserved