Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm. Terminal minihelix and RNA export

The Journal of Biological Chemistry
Carole GwizdekAlain Doglio

Abstract

Determining the cis-acting elements controlling nuclear export of RNA is critical, because they specify which RNA will be selected for transport. We have characterized the nuclear export motif of the adenoviral VA1 RNA, a small cytoplasmic RNA transcribed by RNA polymerase III. Using a large panel of VA1 mutants in both transfected COS cells and injected Xenopus oocytes, we showed that the terminal stem of VA1 is necessary and sufficient for its export. Surprisingly, we found that the nucleotide sequence within the terminal stem is not important. Rather, the salient features of this motif are its length and its relative position within the RNA. Such stems thus define a novel and degenerate cytoplasmic localization motif that we termed the minihelix. This motif is found in a variety of polymerase III transcripts, and cross-competition analysis in Xenopus oocytes revealed that export of one such RNA, like hY1 RNA, is specifically competed by VA1 or artificial minihelix. Taken together these results show that the minihelix defines a new cis-acting export element and that this motif could be exported via a novel and specific nuclear export pathway.

References

Oct 27, 1975·Biochemical and Biophysical Research Communications·B A RoeK Randerath
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M SchwemmleM Bachmann
Feb 20, 1992·Nature·S Piñol-Roma, G Dreyfuss
Nov 1, 1991·Journal of Virology·M B Mathews, T Shenk
Jan 1, 1990·Methods in Enzymology·J Hamm, I W Mattaj
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Khanna-Gupta, V C Ware
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff
Jan 5, 1983·Journal of Molecular Biology·M P Wickens, J B Gurdon
Mar 1, 1994·The Journal of Cell Biology·A JarmolowskiI W Mattaj
Mar 31, 1995·Journal of Molecular Biology·M Zacharias, P J Hagerman
Aug 1, 1995·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·L CagnonA Doglio
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C A O'BrienS L Wolin
Jul 11, 1994·Nucleic Acids Research·C W van GelderG J Pruijn
Aug 13, 1993·Cell·M S Schmidt-ZachmannE A Nigg
Jun 1, 1997·Current Opinion in Cell Biology·S Nakielny, G Dreyfuss
Dec 31, 1997·Methods in Cell Biology·M P Terns, D S Goldfarb
Mar 25, 1998·Current Biology : CB·G J ArtsI W Mattaj
Jun 26, 1998·Current Opinion in Cell Biology·J E Dahlberg, E Lund
Jul 14, 1998·Molecular Cell·U KutayD Görlich
Oct 6, 1998·Annual Review of Biochemistry·I W Mattaj, L Englmeier
Oct 17, 1998·Molecular and Cellular Biology·K HellmuthG Simos
Nov 5, 1998·Molecular Biology of the Cell·S Sarkar, A K Hopper
Nov 10, 1998·Genes & Development·F Stutz, M Rosbash
Dec 19, 1998·The EMBO Journal·G J ArtsI W Mattaj
May 18, 1999·The EMBO Journal·N CustódioM Antoniou
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SarkarA K Hopper
May 12, 2000·Journal of Structural Biology·H GrosshansE Hurt

❮ Previous
Next ❯

Citations

May 12, 2004·Trends in Cell Biology·V Narry Kim
Nov 5, 2002·Trends in Biotechnology·Michael Famulok, Sandeep Verma
Jun 1, 2006·Nature Genetics·Bryan R Cullen
Feb 8, 2011·Nature Structural & Molecular Biology·Yamina BennasserMonsef Benkirane
Nov 7, 2003·Nucleic Acids Research·Eugenia BasyukEdouard Bertrand
Sep 10, 2004·Nucleic Acids Research·Yan Zeng, Bryan R Cullen
Jul 16, 2004·Molecular and Cellular Biology·Ting ChenIan G Macara
Dec 27, 2005·Cell Structure and Function·Takashi MikiYoshihiro Yoneda
Jun 28, 2015·Virus Research·Virginia K Vachon, Graeme L Conn
Jul 15, 2015·The International Journal of Biochemistry & Cell Biology·Madzia P Kowalski, Torsten Krude
Dec 15, 2010·Current Opinion in Structural Biology·Soo Jae LeeTomitake Tsukihara
Feb 7, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Toshihiko TakeiwaMutsuhito Ohno
Nov 21, 2012·Critical Reviews in Biochemistry and Molecular Biology·Emily F Finnegan, Amy E Pasquinelli
Dec 22, 2004·Molecular Cell·Bryan R Cullen
Apr 17, 2002·The Journal of Biological Chemistry·Annie MouginThoru Pederson
Oct 23, 2003·The Journal of Biological Chemistry·Carole GwizdekCatherine Dargemont
Jan 2, 2003·The Journal of Biological Chemistry·Carole GwizdekCatherine Dargemont
Jan 23, 2003·Journal of Cell Science·Bryan R Cullen
Jan 21, 2003·Genes & Development·Anita K Hopper, Eric M Phizicky

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.