Terpene profiling, transcriptome analysis and characterization of cis -β-terpineol synthase from Ocimum

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Atul AnandAshok P Giri

Abstract

Ocimum species produces a varied mix of different metabolites that imparts immense medicinal properties. To explore this chemo-diversity, we initially carried out metabolite profiling of different tissues of five Ocimum species and identified the major terpenes. This analysis broadly classified these five Ocimum species into two distinct chemotypes namely, phenylpropanoid-rich and terpene-rich. In particular, β-caryophyllene, myrcene, limonene, camphor, borneol and selinene were major terpenes present in these Ocimum species. Subsequently, transcriptomic analysis of pooled RNA samples from different tissues of Ocimum gratissimum, O. tenuiflorum and O. kilimandscharicum identified 38 unique transcripts of terpene synthase (TPS) gene family. Full-length gene cloning, followed by sequencing and phylogenetic analysis of three TPS transcripts were carried out along with their expression in various tissues. Terpenoid metabolite and expression profiling of candidate TPS genes in various tissues of Ocimum species revealed spatial variances. Further, putative TPS contig 19414 (TPS1) was selected to corroborate its role in terpene biosynthesis. Agrobacterium-mediated transient over-expression assay of TPS1 in the leaves of O. kilimandsch...Continue Reading

References

Jul 1, 1995·The Plant Cell·D J McGarvey, R Croteau
Jan 23, 1999·Current Opinion in Structural Biology·C A LesburgD W Christianson
Apr 21, 1999·Current Opinion in Biotechnology·R A Dixon
Nov 6, 2001·Applied Microbiology and Biotechnology·S Jennewein, R Croteau
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J RynkiewiczD W Christianson
Jul 29, 2004·Current Pharmaceutical Design·M Rodríguez-Concepción
Dec 7, 2006·Journal of the American Chemical Society·Hirekodathakallu V Thulasiram, C Dale Poulter
Oct 2, 2009·Phytochemistry·Jörg DegenhardtJonathan Gershenzon
May 5, 2011·Natural Product Reports·Dean J Tantillo
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 30, 2013·The International Journal of Developmental Biology·Benoît Lacroix, Vitaly Citovsky
Mar 24, 2016·Biochemical and Biophysical Research Communications·Ramesha H JayaramaiahAshok P Giri

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Datasets Mentioned

BETA
MH925775
MH925776
MH925777

Methods Mentioned

BETA
RNA-Seq

Software Mentioned

ExPASy
KAAS ( KEGG Automatic Annotation Server
CLUSTALW2
MEGA
CAP3
ClustalX
Virtual Ribosome
Bioedit
BLASTX
Velvet

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