TERT mRNA Expression as a Novel Prognostic Marker in Papillary Thyroid Carcinomas

Thyroid : Official Journal of the American Thyroid Association
Aya TanakaNorisato Mitsutake

Abstract

Background: Telomerase reverse transcriptase (TERT) promoter mutations have been found in a subset of papillary thyroid carcinomas (PTCs) and are associated with tumor aggressiveness and worse prognosis. However, little is known about the status of TERT mRNA expression and its relationship between TERT promoter mutations and clinicopathological features. Methods: We analyzed 159 PTC samples for TERT promoter mutations using direct DNA sequencing. TERT expression was measured using quantitative reverse transcription polymerase chain reaction. To examine low allelic frequency of TERT promoter mutations with high sensitivity, we used droplet digital polymerase chain reaction (ddPCR). The relationship between the status of the TERT promoter mutation/expression and clinicopathological features including recurrence risk was statistically analyzed. Results: TERT promoter mutations were found in 20 cases (12.6%). However, TERT expression was observed not only in the mutation-positive tumors but also in 56 of 139 (40.3%) mutation-negative tumors. Among them, we detected low allelic frequency of TERT promoter mutations in three samples (5.4%) using ddPCR. We confirmed a significant association between TERT promoter mutations and aggr...Continue Reading

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Aug 28, 2020·Frontiers in Endocrinology·Brittany A McKelveyMartha A Zeiger
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Jul 30, 2021·Clinical Endocrinology·Tomoe NakaoNorisato Mitsutake

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Methods Mentioned

BETA
reverse transcription PCR
PCR

Software Mentioned

SAS Studio
SPSS Statistics
SAS

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