PMID: 8586613May 1, 1995Paper

Tertiary structure of [2Fe-2S] ferredoxin from Spirulina platensis refined at 2.5 A resolution: structural comparisons of plant-type ferredoxins and an electrostatic potential analysis

Journal of Biochemistry
K FukuyamaH Matsubara

Abstract

The structure of plant-type [2Fe-2S] ferredoxin isolated from Spirulina platensis has been refined using diffraction data to 2.5 A resolution by alternate cycles of simulated annealing and manual revision of the model. The final R factor is 19.9% for 2,912 reflections with F > 2 sigma F between 8.0 and 2.5 A resolution. S. platensis ferredoxin, like other plant-type [2Fe-2S] ferredoxins, has a major alpha-helix flanking a sheet consisting of four beta strands. The present refinement revises the conformation of residues 56-71, in which a one-turn helix was identified. Superposition of the Spirulina ferredoxin structure on the structures of other ferredoxins that have been well refined showed structural perturbation at a few residues on the amino and carboxyl termini and the turn between the first and second beta-strands. The root-mean-square deviations of the corresponding C alpha atoms of the pairs of ferredoxins range from 0.90 to 1.17 A for all the residues, but from 0.64 to 0.70 A if the few perturbed residues are excluded. Therefore, it may be concluded that the main-chain foldings of all the plant-type [2Fe-2S] ferredoxins are essentially the same. Electrostatic potential analysis showed that the molecular surface around t...Continue Reading

Citations

Jun 7, 2013·Metallomics : Integrated Biometal Science·Inmaculada Yruela
Sep 1, 1996·Protein Science : a Publication of the Protein Society·M Vidakovic, J P Germanas
Oct 9, 2013·Applied Biochemistry and Biotechnology·Yong JiaChuan-Chao Dai
Jan 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rachel NechushtaiPatricia A Jennings
Jul 19, 2000·Proteins·A V GrinbergR Bernhardt
Sep 24, 2004·Journal of Molecular Recognition : JMR·Miguel Prudêncio, Marcellus Ubbink
Oct 24, 1998·Progress in Biophysics and Molecular Biology·H Sticht, P Rösch
Dec 23, 2008·The Journal of Physical Chemistry. a·Derrick R J KollingSergei A Dikanov
Jun 8, 2002·Journal of Molecular Biology·Chetlen R CrossnoeKurt L Krause
Jun 18, 2005·Journal of Molecular Biology·Alexander FishOded Livnah
Nov 7, 1996·Chemical Reviews·Richard H. HolmEdward I. Solomon
Nov 7, 1996·Chemical Reviews·P. J. StephensA. Warshel

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