Testicular development and fertility of mice treated prenatally with N-nitroso-N-ethylurea at various gestational stages

Teratogenesis, Carcinogenesis, and Mutagenesis
T NagaoK Imai

Abstract

N-nitroso-N-ethylurea (ENU) was injected intraperitoneally (i.p.) into ICR female mice at 50 mg/kg on day 8, 10, 12, or 16 of gestation (plug day = day 0). Male newborns treated prenatally with ENU were obtained. Body and testes weights of males were measured on postnatal days 4, 12, and 21 and at 12 weeks of age, as well as histopathological observation of their testes. The treated males were mated at 10 weeks to untreated females of the same strain. Subsequently, the plasma testosterone concentration in each group was determined by enzyme immunoassay. Body weight on postnatal day 4 in the group treated on day 16 of gestation was significantly lower compared to that of the control group. On postnatal day 21 and at 12 weeks of age, body weight was significantly lower in all groups treated with ENU compared to that of the control group. Among the embryonic stages tested, embryonic day 10 is the most susceptible to ENU insult with respect to the postnatal development of testes and epididymides, when judged by the relative weight at 12 weeks of age. The fertility of the male offspring was drastically impaired by the prenatal ENU treatment on embryonic day 10, followed by day 12, while the fertility of male offspring treated on emb...Continue Reading

Citations

Jun 15, 2000·Environmental Health Perspectives·J L PryorB Robaire

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