Testicular microbiome in azoospermic men-first evidence of the impact of an altered microenvironment

Human Reproduction
Massimo AlfanoAndrea Salonia

Abstract

Given the relevant role of the extracellular microenvironment in regulating tissue homeostasis, is testicular bacterial microbiome (BM) associated with germ cell aplasia in idiopathic non-obstructive azoospermia (iNOA)? A steady increase of dysbiosis was observed among testis with normal spermatogenesis vs. iNOA with positive sperm retrieval and iNOA with complete germ cell aplasia. Tissue-associated BM has been reported to be a biologically important extracellular microenvironment component for numerous body habitats, but not yet for the human testis. Cross-sectional study, investigating tissue-associated BM in the testis of (i) five men with iNOA and negative sperm retrieval at microdissection testicular sperm extraction (microTESE); (ii) five men with iNOA and positive sperm retrieval at microTESE; and (iii) five normozoospermic men upon orchiectomy. Every testicular specimen was histologically classified and analyzed in terms of bacterial community. Massive ultra-deep pyrosequencing was applied to investigate testis microbiome. Metagenome was analyzed using Quantitative Insights Into Microbial Ecology (QIIME). Tissue-associated bacterial load was quantified by digital droplet PCR. Normozoospermic men showed small amounts of...Continue Reading

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Citations

Jul 14, 2019·International Urology and Nephrology·Joseph K M LiChi-Fai Ng
Apr 3, 2020·Current Opinion in Urology·Scott D LundyRanjith Ramasamy
May 31, 2019·Communicative & Integrative Biology·William B Miller, John S Torday
Nov 17, 2019·Nature Reviews. Urology·Signe AltmäeReet Mändar
Dec 12, 2020·Journal of Gynecology Obstetrics and Human Reproduction·O TsonisM Paschopoulos
Mar 23, 2021·Current Sexual Health Reports·Vadim OsadchiySriram V Eleswarapu
Mar 31, 2021·Journal of Equine Veterinary Science·C Giselle CookeJoanna E Harnett
Nov 15, 2020·European Journal of Obstetrics, Gynecology, and Reproductive Biology·G A CorbettF M McAuliffe
Aug 5, 2021·Reproductive Biomedicine Online·Nerea M MolinaSigne Altmäe
Aug 28, 2021·Communications Biology·Laura Martinez-VidalMassimo Alfano
Dec 18, 2021·Andrology·Yan Wang, Zuogang Xie
Feb 4, 2022·Journal of Endocrinological Investigation·M A VenneriA Lenzi

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