Abstract
The FilmArray Respiratory Panel (RP) detects multiple pathogens, including Bordetella pertussis. The multiplex PCR system is appropriate for a core laboratory or point of care due to ease of use. The purpose of this study is to compare the analytical sensitivity of the FilmArray RP, which targets the promoter region of the B. pertussis toxin gene, with the Focus real-time PCR assay, which targets the insertion sequence IS481. Seventy-one specimens from patients aged 1 month to 18 years, which had tested positive for B. pertussis using the Focus assay, were analysed using the FilmArray RP. Forty-six specimens were positive for B. pertussis by both the Focus and the FilmArray RP assays. Twenty-five specimens were negative for B. pertussis using the FilmArray RP assay, but positive using the Focus assay. The FilmArray RP assays will detect approximately 1/3 less cases of B. pertussis than the Focus assay.
References
Sep 1, 1990·Journal of Clinical Microbiology·E M GlareI T Nisbet
Apr 28, 2001·Journal of Clinical Microbiology·U ReischlM J Loeffelholz
Dec 8, 2006·Journal of Clinical Microbiology·Xuan QinJanet A Englund
Aug 20, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Yinghua XuShumin Zhang
Sep 24, 2011·Journal of Clinical Microbiology·Kathleen M TattiMaria Lucia Tondella
Nov 1, 2011·PloS One·Mark A PoritzKirk M Ririe
Nov 26, 2011·Journal of Clinical Microbiology·Virginia M PierceRichard L Hodinka
Aug 10, 2012·Epidemiology and Infection·D BotteroD F Hozbor
Aug 14, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A TizolovaS Guillot
Oct 23, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Loren RodgersMary Diorio
Feb 21, 2013·Journal of Clinical Laboratory Analysis·Marc Roger CouturierJudy A Daly
Citations
Dec 23, 2015·Expert Review of Molecular Diagnostics·Stacy G BealKenneth H Rand
Nov 3, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·María Esther CastilloJuana Del Valle Mendoza
Nov 7, 2015·Paediatric Drugs·Abdulbaset M SalimPaul E Kilgore
Dec 8, 2017·Journal of Clinical Microbiology·N Esther BabadyWallace Greene
Mar 30, 2018·Journal of Clinical Microbiology·Amy L LeberBart Kensinger
Apr 29, 2016·Journal of Clinical Microbiology·Yi-Wei TangHongwei Zhang
May 8, 2019·Critical Reviews in Clinical Laboratory Sciences·Deirdre L Church, Christopher Naugler
Mar 7, 2020·Journal of Clinical Microbiology·Amy L LeberIrene Hannet
Feb 23, 2019·Journal of Clinical Microbiology·T McMillenN E Babady
Aug 15, 2020·Open Forum Infectious Diseases·Sophie GuillotJulie Toubiana
Jul 4, 2020·BMC Infectious Diseases·María Camila Arango-Granados, Iván Mauricio Trompa
Dec 12, 2020·Journal of General and Family Medicine·Satoshi SuzukiTetsuhiro Maeno
Dec 11, 2020·The Journal of Emergency Medicine·Colleen E MayhewAllison D Cator
Feb 19, 2021·Journal of Clinical Microbiology·Linoj P SamuelUNKNOWN ASM Clinical and Public Health Microbiology Committee
Aug 28, 2021·BMC Microbiology·Suzanne A V van AstenEric C J Claas