Testing of Arg-8-gonadotropin-releasing hormone-directed antisera by immunological and immunocytochemical methods for use in comparative studies.

Histochemistry
S BlähserW J Kuenzel

Abstract

Three polyclonal antisera raised in rabbits against the mammalian molecular form of gonadotropin-releasing hormone (GnRH) were tested in enzyme-linked immunosorbent assays for crossreactivity with naturally occurring GnRHs and with GnRH analogues. Antisera were then tested immunocytochemically in order (i) to identify amino acids essential for the binding of each antiserum, and (ii) to evaluate the specificity of the immunocytochemical reaction in brain sections from various species of cyclostomes, amphibians, reptiles, and birds. Antiserum GnRH 80/1, recognizing mainly a discontinuous determinant including the NH2- and COOH-termini, crossreacts with GnRHs the molecular bending of which enables the spatial approach of both terminal amino acid residues. Antiserum GnRH 80/2, by requiring the COOH-terminus for binding and not tolerating substitutions by aromatic amino acids in the middle region of the molecule, recognizes chicken I GnRH, however, not the salmon form. The use of this antiserum is appropriate in species synthesizing the mammalian and/or the chicken I form of GnRH. GnRH antiserum 81/1 is specific mostly for mammalian GnRH. The results obtained by ELISAs are confirmed by immunocytochemical studies. A comparison betwee...Continue Reading

References

Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger
Oct 15, 1986·Brain Research·C R Wirsig, T V Getchell
Mar 1, 1985·Peptides·R C PowellR P Millar
Jun 18, 1971·Biochemical and Biophysical Research Communications·H MatsuoA V Schally
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N SherwoodW Vale

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Citations

Apr 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ishwar S ParharYasuo Sakuma
May 26, 1995·Pharmacy World & Science : PWS·A L Wertheimer, K B Andrews
Dec 23, 2020·Open Biology·Guillaume Poncelet, Sebastian M Shimeld

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