Testing the utility of fluorescent proteins in Mimulus lewisii by an Agrobacterium-mediated transient assay

Plant Cell Reports
Baoqing Ding, Yao-Wu Yuan

Abstract

The Agrobacterium -mediated transient expression assay by leaf infiltration in Mimulus lewisii is robust. Fluorescent proteins EGFP, EYFP and DsRed give bright fluorescence signals in the infiltrated tissue. Mimulus lewisii is an emerging developmental genetic model system. Recently developed genomic and genetic resources and a stable transformation protocol have greatly facilitated the identification and functional characterization of genes controlling the development of ecologically important floral traits using this species. To further expedite gene and protein function analyses in M. lewisii, we adopted and simplified the Agrobacterium-mediated transient gene expression method routinely used in tobacco plants. With the validated transient assay, we examined the performance of fluorescent proteins EGFP, EYFP and DsRed in M. lewisii. All three proteins gave bright fluorescence signals when transiently expressed in agroinfiltrated leaves. Furthermore, we demonstrated the utility of fluorescent proteins in M. lewisii by showing the nuclear localization of Reduced Carotenoid Pigmentation 1 (RCP1), a recently discovered R2R3-MYB transcription factor that regulates carotenoid pigmentation during flower development. Both the transi...Continue Reading

References

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Aug 12, 2014·The New Phytologist·Yao-Wu YuanHarvey D Bradshaw
Sep 18, 2015·The New Phytologist·Janelle M SagawaYao-Wu Yuan

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Citations

Aug 25, 2019·International Journal of Molecular Sciences·Hongqiu ZengHaitao Shi
Apr 26, 2021·The Plant Cell·Taliesin J KinserJoshua R Puzey

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