Testis-specific expression of a functional retroposon encoding glucose-6-phosphate dehydrogenase in the mouse

Genomics
P J HendriksenJ A Grootegoed

Abstract

The X-chromosomal gene glucose-6-phosphate dehydrogenase (G6pd) is known to be expressed in most cell types of mammalian species. In the mouse, we have detected a novel gene, designated G6pd-2, encoding a G6PD isoenzyme. G6pd-2 does not contain introns and appears to represent a retroposed gene. This gene is uniquely transcribed in postmeiotic spermatogenic cells in which the X-encoded G6pd gene is not transcribed. Expression of the G6pd-2 sequence in a bacterial system showed that the encoded product is an active enzyme. Zymogramic analysis demonstrated that recombinant G6PD-2, but not recombinant G6PD-1 (the X-chromosome-encoded G6PD), formed tetramers under reducing conditions. Under the same conditions, G6PD tetramers were also found in extracts of spermatids and spermatozoa, indicating the presence of G6pd-2-encoded isoenzyme in these cell types. G6pd-2 is one of the very few known expressed retroposons encoding a functional protein, and the presence of this gene is probably related to X chromosome inactivation during spermatogenesis.

References

Jun 1, 1992·Human Molecular Genetics·M TownL Luzzatto
Nov 1, 1992·Developmental Biology·J R McCarreyJ J Rossi
Feb 13, 1992·Biochimica Et Biophysica Acta·J M BautistaL Luzzatto
May 7, 1992·Biochimica Et Biophysica Acta·J FitzgeraldH H Dahl
Apr 1, 1991·Trends in Genetics : TIG·P KoopmanR Lovell-Badge
Sep 1, 1990·Somatic Cell and Molecular Genetics·R M BrownG K Brown
Feb 25, 1990·Nucleic Acids Research·J R McCarrey
Feb 25, 1990·Nucleic Acids Research·J ZimmermannW Ansorge
Apr 1, 1990·The Journal of Experimental Zoology·P Ninfali, F Palma
Aug 1, 1985·Molecular and Cellular Biology·M B SoaresA Efstratiadis
Sep 1, 1987·Molecular and Cellular Biology·P H BoerM W McBurney
Dec 1, 1988·European Journal of Biochemistry·P J MasonL Luzzatto
Feb 1, 1988·Biochemical Genetics·W PretschS Merkle
Aug 11, 1988·Nucleic Acids Research·Y S HoJ D Crapo
Feb 28, 1985·Biochemical and Biophysical Research Communications·R N MartinsC L Masters
Jan 1, 1985·Annual Review of Genetics·E F Vanin
Dec 1, 1971·Archives of Biochemistry and Biophysics·A BonsignoreA De Flora
Jan 1, 1974·International Review of Cytology·A J Solari
Jan 20, 1967·Science·W M Fitch, E Margoliash
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Aug 26, 1966·Science·S OhnoE Beutler
Jun 1, 1980·European Journal of Biochemistry·C Auffray, F Rougeon
Apr 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S J ConwayP S Burgoyne
Aug 1, 1995·Developmental Biology·P J HendriksenJ A Grootegoed
Oct 1, 1994·Molecular Reproduction and Development·R C IannelloI Kola
Aug 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M W McBurneyL Pickavance
Apr 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Aitken, H Fisher

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Citations

Jul 20, 2004·Nature Genetics·Julie BradleyDavid C Page
Dec 20, 2002·Nucleic Acids Research·Tamayo UechiNaoya Kenmochi
Aug 25, 2005·Cold Spring Harbor Symposia on Quantitative Biology·K D Huynh, J T Lee
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott W RoyWalter Gilbert
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A M WallaceC C MacDonald
Sep 23, 2003·Molecular Reproduction and Development·Carmine PittoggiCorrado Spadafora
Feb 20, 2007·Journal of Evolutionary Biology·P GayralN Galtier
Dec 1, 2000·Baillière's Best Practice & Research. Clinical Endocrinology & Metabolism·J A GrootegoedW M Baarends
Nov 9, 2010·Gynecologic Oncology·Henry L ChangPatricia K Donahoe
Sep 11, 2001·The Journal of Experimental Zoology·A FarazmandP K Basrur
Apr 9, 2005·Nature Reviews. Genetics·Khanh D Huynh, Jeannie T Lee
Nov 10, 2018·Annual Review of Animal Biosciences·Wan-Sheng Liu
Mar 29, 2000·Theriogenology·P J Hendriksen
Apr 25, 2003·Trends in Genetics : TIG·Chung I Wu, Eugene Yujun Xu
Mar 24, 2004·Trends in Endocrinology and Metabolism : TEM·P Jeremy Wang

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