PMID: 7358215Jan 1, 1980

Testosterone 5 alpha-reductase activity of skin fibroblasts. Increase with serial subcultures

Molecular and Cellular Endocrinology
I MowszowiczP Mauvais-Jarvis

Abstract

Cultures of skin fibroblasts from different anatomical sites have been established and testosterone 5 alpha-reductase assayed after the 2nd, 6th and 12th subcultures. After the 2nd passage, 5 alpha-reduction of testosterone to both dihydrotestosterone and androstanediols correlated well with that measured in direct assays performed in total skin homogenates; this is an additional evidence that different types of skin have specific levels of testosterone 5 alpha-reductase activity. However, there was a marked increase in 5 alpha-reductase activity with successive subcultures (X4--5 between 2nd and 12th subcultures) in all the cell strains studied. This finding could explain why data concerning 5 alpha-reductase in cultured skin fibroblasts are often conflicting with those in skin homogenates. It emphasizes the necessity of performing enzyme assays after the same number of passages to allow comparison from strain to strain. Different hypotheses are discussed in order to explain such variations of 5 alpha-reductase in cultured human skin fibroblasts.

References

Sep 1, 1978·The Journal of Clinical Investigation·M LeshinJ D Wilson
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W J MeyerC J Migeon
Mar 1, 1977·Primary Care·L B Morrow
Aug 18, 1977·The New England Journal of Medicine·J A AmrheinC J Migeon
Jul 1, 1977·Molecular and Cellular Endocrinology·I Mowszowicz, C W Bardin
Jun 1, 1972·The Journal of Clinical Investigation·D D ShanieM I New

Citations

Apr 13, 1981·Molecular and Cellular Biochemistry·T R Brown, C J Migeon
Jul 1, 1982·Journal of Steroid Biochemistry·C Vallet-StrouveI Mowszowicz
Jul 1, 1986·Journal of Steroid Biochemistry·A ChababC Sultan
Dec 1, 1987·Molecular and Cellular Endocrinology·D StamatiadisP Mauvais-Jarvis
Feb 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·I MorimotoT Hara
Jan 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·D StamatiadisP Mauvais-Jarvis
Feb 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·M BlanchèreI Mowszowicz
Sep 28, 1998·The Journal of Steroid Biochemistry and Molecular Biology·M BlanchèreI Mowszowicz
Jul 12, 2002·International Journal of Urology : Official Journal of the Japanese Urological Association·Kazumi NakaeHiroki Shima
Dec 15, 1984·American Journal of Obstetrics and Gynecology·C SultanR Jean
May 1, 1986·Clinics in Endocrinology and Metabolism·P Mauvais-Jarvis

Related Concepts

Cell Division Phases
Fibroblasts
Hirsutism
Oxidase
Glans Penis
Skin
Reifenstein Syndrome
Testosterone 5-alpha-Reductase

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