PMID: 6403672May 1, 1983Paper

Testosterone 5 alpha-reductase in rat brain

Journal of Neurochemistry
N R KriegerM E Jurman

Abstract

We describe a simple procedure for the microassay of testosterone 5 alpha-reductase in homogenates of rat brain. This enzyme converts testosterone to dihydrotestosterone. We have used this assay to characterize the enzymatic activity and to map its distribution. The apparent Km is 4.1 x 10(-6) M and the Vmax is 85.6 pmol/mg protein/h. The pH optimum is broad and extends from pH 6.0 to 8.0. For the brain regions surveyed, testosterone 5 alpha-reductase activity varied over a 10-fold range. The highest activities were observed in homogenates of the midbrain and pons (37-39 pmol/mg protein/h). The lowest were seen in homogenates of the thalamus, caudate nucleus, frontal cortex, hippocampus, hypothalamus, olfactory tubercle, and preoptic area (3-7 pmol/mg protein/h).

References

Jul 1, 1978·The Journal of Endocrinology·A I LeshnerE Terasawa
Jan 1, 1979·Annual Review of Neuroscience·B S McEwenD W Pfaff
May 1, 1978·Journal of Steroid Biochemistry·M Ginsburg, D K Shori
Dec 1, 1978·Pharmacology, Biochemistry, and Behavior·A I LeshnerT B van Wimersma Greidanus
Feb 15, 1974·Science·B W O'Malley, A R Means
Sep 15, 1973·The Journal of Comparative Neurology·D Pfaff, M Keiner
Dec 1, 1973·The Journal of Endocrinology·C DenefB S McEwen
Mar 20, 1981·Science·N J MacLusky, F Naftolin
Mar 20, 1981·Science·B S McEwen
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S D ErulkarN R Krieger
Mar 1, 1982·Journal of Neurochemistry·M E JurmanN R Krieger
Dec 1, 1980·Hormones and Behavior·S D Holman, R W Goy

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Citations

Aug 1, 1986·Journal of Steroid Biochemistry·U Enderle-SchmittG Aumüller
Mar 1, 1987·Journal of Steroid Biochemistry·M Seidl, K M Pirke
Apr 1, 1988·Journal of Steroid Biochemistry·J A ReskoC E Roselli
Aug 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·P Negri-CesiF Celotti
Sep 1, 1996·Steroids·T M PenningM Lewis
Oct 19, 2002·Progress in Neurobiology·Roberto C MelcangiMariarita Galbiati
Dec 1, 1993·Journal of Neurochemistry·A KorneyevE Costa
Jul 31, 2007·Brain Research Reviews·Michael J WeiserRobert J Handa
Nov 23, 2019·Journal of Neurochemistry·Hillary L CanslerDaniel W Wesson
Apr 15, 1989·International Journal of Cancer. Journal International Du Cancer·M MaasbergK Havemann
Mar 1, 1984·Journal of Neurochemistry·N R Krieger, R G Scott
Sep 29, 1987·Brain Research·N J MacLuskyF Naftolin

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