Testosterone induces renal tubular epithelial cell death through the HIF-1α/BNIP3 pathway.

Journal of Translational Medicine
Yonghan PengYinghao Sun

Abstract

The morbidity of nephrolithiasis is 2-3 times higher in males than in females, suggesting that androgen plays a key role in nephrolithiasis. The death of renal tubular epithelial cells (TECs) is an important pathophysiological process contributing to the development of nephrolithiasis. Therefore, the aim of this study is to investigate whether androgen directly induces TECs apoptosis and necrosis and its underlying mechanisms in kidney stone formation. We compared serum testosterone level between male and female healthy volunteers and kidney stone patients. The in vivo nephrolithiasis model was established using glyoxylic acid, and calcium deposits were detected by van Kossa staining. In the in vitro study using mouse TECs (TCMK-1 cells) and human TECs (HK-2 cells), apoptosis, necrosis, and the expression of BH3-only protein Bcl-2-like 19 kDa-interacting protein 3 (BNIP3) were examined incubated with different doses of testosterone using flow cytometry. Levels of apoptosis-related proteins transfected with the BNIP3 siRNA were examined by western blotting. The mitochondrial potential (ΔΨm) was detected by JC-1 staining and flow cytometry. We monitored BNIP3 expression in the testosterone-induced TECs injury model after treatmen...Continue Reading

References

Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·R K Bruick
Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
Apr 17, 2004·Kidney International·Daniela VerzolaGiacomo Garibotto
Feb 16, 2005·The Journal of Urology·Margaret S PearleUNKNOWN Urologic Diseases of America Project
Jun 1, 1937·Annals of Surgery·A Randall
Feb 14, 2008·International Journal of Urology : Official Journal of the Japanese Urological Association·Masao Tsujihata
Jul 10, 2009·Autophagy·Nathalie M Mazure, Jacques Pouysségur
Jul 21, 2009·Biochemical and Biophysical Research Communications·Daniela VerzolaGiacomo Garibotto
Oct 19, 2010·The Journal of Urology·Jin-Yi LiJianguo Hou
May 23, 2013·Journal of Neurochemistry·Chengren LiJiming Kong
May 16, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Bin WangFei Zou
Jan 29, 2016·Nature Communications·Shrikant R MulayHans-Joachim Anders
Apr 30, 2017·Seminars in Cancer Biology·Lauren E DrakeKay F Macleod
Feb 1, 2018·International Journal of Molecular Sciences·Joshua M KaplanSean Dikdan
Mar 28, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Irene FilippiAntonella Naldini

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Citations

Nov 3, 2020·American Journal of Physiology. Renal Physiology·Regina F SultanovaDaria V Ilatovskaya
Feb 14, 2021·Clinical and Translational Science·Junxiang WenJian Li
Nov 17, 2020·Frontiers in Physiology·Sakdithep Chaiyarit, Visith Thongboonkerd
Dec 29, 2020·Journal of Cellular and Molecular Medicine·Wenting YeLili Zhou
Apr 14, 2021·Journal of Translational Medicine·Yonghan PengYinghao Sun
Sep 28, 2021·Journal of Animal Physiology and Animal Nutrition·Lewei GuoJun Wang

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Methods Mentioned

BETA
Assay
flow cytometry
immunoprecipitation
transfection
transfections
ChIP

Software Mentioned

Image
Pro Plus
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis