Testosterone induces vascular smooth muscle cell migration by NADPH oxidase and c-Src-dependent pathways

Hypertension
Andreia Z ChignaliaRita C Tostes

Abstract

Testosterone has been implicated in vascular remodeling associated with hypertension. Molecular mechanisms underlying this are elusive, but oxidative stress may be important. We hypothesized that testosterone stimulates generation of reactive oxygen species (ROS) and migration of vascular smooth muscle cells (VSMCs), with enhanced effects in cells from spontaneously hypertensive rats (SHRs). The mechanisms (genomic and nongenomic) whereby testosterone induces ROS generation and the role of c-Src, a regulator of redox-sensitive migration, were determined. VSMCs from male Wistar-Kyoto rats and SHRs were stimulated with testosterone (10(-7) mol/L, 0-120 minutes). Testosterone increased ROS generation, assessed by dihydroethidium fluorescence and lucigenin-enhanced chemiluminescence (30 minutes [SHR] and 60 minutes [both strains]). Flutamide (androgen receptor antagonist) and actinomycin D (gene transcription inhibitor) diminished ROS production (60 minutes). Testosterone increased Nox1 and Nox4 mRNA levels and p47phox protein expression, determined by real-time PCR and immunoblotting, respectively. Flutamide, actinomycin D, and cycloheximide (protein synthesis inhibitor) diminished testosterone effects on p47phox. c-Src phosphoryl...Continue Reading

References

Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J I JonesD R Clemmons
Jan 16, 1998·Andrologia·G B ChainyL Samanta
Oct 30, 1999·Canadian Journal of Physiology and Pharmacology·Y OdaM D Hollenberg
Jul 17, 2001·Biochemical and Biophysical Research Communications·Z WangW H Newman
Nov 7, 2001·Coronary Artery Disease·U Landmesser, D G Harrison
Sep 10, 2002·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F L DavidR C A Tostes
Jan 22, 2004·Journal of Molecular and Cellular Cardiology·Zhongbiao WangWalter H Newman
Apr 8, 2004·Biological Research for Nursing·Marguerite Littleton-Kearney, Patricia D Hurn
Nov 10, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Howard I Scher, Charles L Sawyers
Dec 20, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Maria A CavasinXiao-Ping Yang
Jun 17, 2008·Metabolism: Clinical and Experimental·Maria Augusta MaturanaPoli Mara Spritzer
Jan 29, 2010·American Journal of Physiology. Cell Physiology·Caroline Serrano-NascimentoMaria Tereza Nunes
Mar 12, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Norma B OjedaBarbara T Alexander
Jul 2, 2010·The New England Journal of Medicine·Shehzad BasariaShalender Bhasin
Dec 15, 2010·Geriatrics & Gerontology International·Shiho FukaiYasuyoshi Ouchi
Oct 21, 2011·The Journal of Clinical Endocrinology and Metabolism·Zoë HydeBu B Yeap

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Citations

Aug 10, 2012·American Journal of Physiology. Cell Physiology·Katherine J MasseyJeffrey L Garvin
May 9, 2012·Hypertension·Matthias BartonMatthias R Meyer
Aug 2, 2013·Antioxidants & Redox Signaling·Elisa Giannoni, Paola Chiarugi
Jul 3, 2013·Physiology·Margaret A Zimmerman, Jennifer C Sullivan
Dec 24, 2014·International Journal of Molecular Sciences·Marius C StaiculescuLuis A Martinez-Lemus
Jun 19, 2013·The Journal of Surgical Research·Deidra J H MountainOscar H Grandas
Feb 25, 2014·Journal of Molecular and Cellular Cardiology·Ralf P BrandesKatrin Schröder
Aug 30, 2016·Vascular Pharmacology·Agostino VirdisStefano Taddei
Aug 6, 2015·Clinical Medicine Insights. Endocrinology and Diabetes·Entesar Oa El SaghierAbdelnasser Gharib
Aug 28, 2013·Hypertension·Kate M DentonMarianne Tare
Mar 25, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Rheure Alves Moreira LopesRita C Tostes
Feb 1, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Tiago J CostaMaria Helena C Carvalho
Aug 10, 2017·Canadian Journal of Physiology and Pharmacology·Leonardo Souza-SilvaVictor Vitorino Lima
Jun 25, 2017·Clinical Science·Angela K Lucas-HeraldRhian M Touyz
Sep 26, 2013·The Journal of Clinical Endocrinology and Metabolism·Johannes B RuigeJean-Marc Kaufman
Nov 6, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rita C TostesJane F Reckelhoff
Jul 25, 2017·Journal of Molecular Endocrinology·Angelina Rafaela DebortoliRoger Lyrio Dos Santos
Aug 29, 2014·The Journal of Biological Chemistry·Eunbi KoYun Soo Bae
Oct 17, 2020·Journal of the American Heart Association·Yasushi TashimaMichael P Fischbein
Jan 23, 2021·Molecular and Cellular Biochemistry·Taofeek O UsmanLawrence A Olatunji
Jul 28, 2020·The Journal of Steroid Biochemistry and Molecular Biology·Saulo Chaves MagalhãesAriclécio Cunha de Oliveira
Dec 29, 2020·Journal of Cellular and Molecular Medicine·Wenting YeLili Zhou
Jun 4, 2020·Steroids·Fernando A C SearaJose H M Nascimento
Sep 25, 2020·Small GTPases·Kenia Pedrosa Nunes, R Clinton Webb
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lawrence A OlatunjiOlufunto O Badmus
May 19, 2021·Journal of Basic and Clinical Physiology and Pharmacology·Wender do Nascimento RouverRoger Lyrio Dos Santos
Jun 22, 2021·Frontiers in Molecular Biosciences·Hongliang ZhangJinhui Tao
Jul 23, 2021·Cardiovascular Research·Antonio CittadiniAndrea Salzano

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