Testosterone insulin-like effects: an in vitro study on the short-term metabolic effects of testosterone in human skeletal muscle cells

Journal of Endocrinological Investigation
Cristina AntinozziLuigi Di Luigi

Abstract

Testosterone by promoting different metabolic pathways contributes to short-term homeostasis of skeletal muscle, the largest insulin-sensitive tissue and the primary site for insulin-stimulated glucose utilization. Despite evidences indicate a close relationship between testosterone and glucose metabolism, the molecular mechanisms responsible for a possible testosterone-mediated insulin-like effects on skeletal muscle are still unknown. Here we used undifferentiated proliferating or differentiated human fetal skeletal muscle cells (Hfsmc) to investigate the short-term effects of testosterone on the insulin-mediated biomolecular metabolic machinery. GLUT4 cell expression, localization and the phosphorylation/activation of AKT, ERK, mTOR and GSK3β insulin-related pathways at different time points after treatment with testosterone were analyzed. Independently from cells differentiation status, testosterone, with an insulin-like effect, induced Glut4-mRNA expression, GLUT4 protein translocation to the cytoplasmic membrane, while no effect was observed on GLUT4 protein expression levels. Furthermore, testosterone treatment modulated the insulin-dependent signal transduction pathways inducing a rapid and persistent activation of AKT,...Continue Reading

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Citations

Nov 22, 2017·International Journal of Cancer. Journal International Du Cancer·Pedro Gonzalez-MenendezRosa M Sainz
Mar 2, 2018·PloS One·José Vilaça-AlvesVictor Machado Reis
Dec 22, 2017·European Respiratory Review : an Official Journal of the European Respiratory Society·Stephen Y Chan, Lewis J Rubin
Jun 29, 2021·Current Opinion in Pharmacology·Paolo SgròLuigi Di Luigi
Aug 21, 2021·The Journal of Clinical Endocrinology and Metabolism·Maartje KlaverRenée de Mutsert

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Methods Mentioned

BETA
PCR

Software Mentioned

Quantity One
Image Quant Las 4000
GraphPad
Hfsmc
7500
GraphPad Prism

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