PMID: 7082729May 1, 1982Paper

Testosterone metabolism by proximal and distal regions of the vas deferens from immature and mature rats

Biology of Reproduction
L SeethalakshmiA Steinberger


The vas deferens, an androgen-dependent organ, may have physical importance in sperm maturation, yet very little is known about its steroid capabilities. To explore the steroid metabolic activities and their possible changes during sexual maturation, a comparative study of [14C]testosterone metabolism by the proximal (VDP) and distal (VDD) segments of the vas deferens from immature and mature rats was carried out in vitro. Approximately 50 mg of the VDP and VDD portions of the vas deferens, devoid of spermatozoa, were incubated in duplicates for 3 h at 37 degrees C with [14C]testosterone (53.5 microCi/mmol) and the essential cofactors. After the addition of appropriate 3H-labeled recovery tracers to the incubates, the steroids were extracted, separated by paper and thin-layer chromatography and crystallized to constant 3H/14C ratio in 3 consecutive crystals. The results showed that all tissues metabolized [14C]testosterone into 4 major steroids: dihydrotestosterone (DHT), 5 alpha-androstane-3 alpha-17 beta diol (5 alpha-3 alpha-diol), delta 4-androstenedione and androsterone. The metabolites accounted for approximately 16% and approximately 6% of the conversion products in the immature and mature vas deferens respectively. Ther...Continue Reading


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