Apr 13, 1976

Testosterone metabolism in male rat epiphysis

Calcified Tissue Research
R J Ackerman, D W Hamilton


Using radioactive substrate, thin-layer chromatography and recrystallization methods as well as differential centrifugation, gel filtration and electrophoresis, testosterone metabolism was investigated in male rat epiphyseal growth plate. 5alpha-androstane 3alpha-17beta-diol was found to be a major metabolite in vitro; lesser amounts of androstenedione and androstanedione, and a very small amount of 5alpha-dihydrotestosterone were also identified. Radioactivity was recovered in epiphyseal subcellular fractions 30 minutes following in vivo administration of tritiated 5alpha-dihydrotestosterone; within 2 hours radioactivity had fallen to essentially background level in all fractions but the cytosol. A testosterone binding protein could not be identified within epiphyseal cytosol.

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Mentioned in this Paper

Metabolic Process, Cellular
Testosterone Measurement
Dihydrotestosterone Assay
Androstenedione Measurement
Epiphysis Disorders
Plasma Protein Binding Capacity
Testosterone binding protein

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