Testosterone modulates endothelial progenitor cells in rat corpus cavernosum

BJU International
In Sang HwangK H Park

Abstract

To investigate the effects of testosterone on cavernosal endothelial progenitor cells (EPCs) in a castrated rat model. In all, 45 male Sprague-Dawley rats (12-weeks old) were divided into control, surgical castration, and castration with testosterone replacement groups. The rats were castrated under ketamine anaesthesia, and testosterone was administered by daily subcutaneous injection of 3 mg/kg testosterone propionate. The corpus cavernosum was obtained after perfusion with 10 mL saline via the abdominal aorta 4 weeks later. The expression of EPC-specific markers [cluster of differentiation 34 (CD34), fetal liver kinase 1 (Flk1), and vascular endothelial (VE)-cadherin] was evaluated by flow cytometry analysis and immunofluorescence staining. CD34+/Flk1+ and CD34+/VE-cadherin+ cells were detected in the cavernosal sinusoidal endothelial space. Flow cytometry analysis showed that CD34 and Flk1 double positive cells (EPCs) comprised ≈3.79% of the corpus cavernosum in normal rats. The percentage of EPC marker-positive cells decreased significantly in the castration group (2.8%; P < 0.05) and was restored to 3.56% after testosterone supplementation. Confocal microscopy revealed that the numbers of CD34+/Flk1+ and CD34+/VE-cadherin...Continue Reading

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Citations

Oct 2, 2020·Biological Reviews of the Cambridge Philosophical Society·Ogechi OgokeNatesh Parashurama
Apr 10, 2021·Investigative and Clinical Urology·Ho Seok ChungKwangsung Park
Apr 15, 2021·Reproductive Medicine and Biology·Daiki HashimotoGen Yamada

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