Testosterone regulates the autophagic clearance of androgen binding protein in rat Sertoli cells

Scientific Reports
Yi MaXiao-Nan Kang

Abstract

Dysregulation of androgen-binding protein (ABP) is associated with a number of endocrine and andrology diseases. However, the ABP metabolism in Sertoli cells is largely unknown. We report that autophagy degrades ABP in rat Sertoli cells, and the autophagic clearance of ABP is regulated by testosterone, which prolongs the ABP biological half-life by inhibiting autophagy. Further studies identified that the autophagic clearance of ABP might be selectively regulated by testosterone, independent of stress (hypoxia)-induced autophagic degradation. These data demonstrate that testosterone up-regulates ABP expression at least partially by suppressing the autophagic degradation. We report a novel finding with respect to the mechanisms by which ABP is cleared, and by which the process is regulated in Sertoli cells.

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Methods Mentioned

BETA
gene knockdown
Transfection
PCR
confocal microscopy
Enzyme-Linked Immunosorbent Assays
ELISA

Software Mentioned

- Prism5
GraphPad

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