Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction

The Journal of Endocrinology
Fengyue WangLu Fu

Abstract

Testosterone can affect cardiovascular disease, but its effects on mitochondrial dynamics in the post-infarct myocardium remain unclear. To observe the effects of testosterone replacement, a rat model of castration-myocardial infarction (MI) was established by ligating the left anterior descending coronary artery 2 weeks after castration with or without testosterone treatment. Expression of mitochondrial fission and fusion proteins was detected by western blot and immunofluorescence 14 days after MI. Cardiac function, myocardial inflammatory infiltration and fibrosis, cardiomyocyte apoptosis, mitochondrial microstructure, and ATP levels were also assessed. Compared with MI rats, castrated rats showed aggravated mitochondrial and myocardial insults, including mitochondrial swelling and disordered arrangement; loss of cristae, reduced mitochondrial length; decreased ATP levels; cardiomyocyte apoptosis; and impaired cardiac function. Results of western blotting analyses indicated that castration downregulated peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1A) and mitofusin 2, but upregulated dynamin-related protein 1. The results were also supported by results obtained using immunofluorescence. However, t...Continue Reading

References

Sep 28, 2002·Molecular Endocrinology·Cynthia A Heinlein, Chawnshang Chang
Feb 5, 2003·Cardiovascular Research·Matthias NahrendorfFrank Callies
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·E R Simpson
Jan 5, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anne GarnierRenée Ventura-Clapier
Oct 4, 2007·The Journal of Clinical Endocrinology and Metabolism·Gail A LaughlinJaclyn Bergstrom
Jun 4, 2008·Biochimica Et Biophysica Acta·Gilad TwigOrian S Shirihai
Dec 3, 2008·Cardiovascular Research·Masatsugu Hori, Kazuhiko Nishida
Mar 3, 2009·Journal of Molecular and Cellular Cardiology·Sandra SenaSheldon E Litwin
Jun 6, 2009·Cardiovascular Research·Le ChenAnne A Knowlton
Aug 12, 2009·Cardiovascular Research·Lisa C HeatherKieran Clarke
Apr 13, 2010·Trends in Endocrinology and Metabolism : TEM·T H Jones
May 27, 2010·Molecular Endocrinology·Yasumasa IkedaToshio Matsumoto
Jun 9, 2010·The Journal of Clinical Endocrinology and Metabolism·M Mercè Fernández-BalsellsVictor M Montori
Jul 2, 2010·The New England Journal of Medicine·Shehzad BasariaShalender Bhasin
Jul 16, 2010·Cardiovascular Research·Sang-Bing Ong, Derek J Hausenloy
Oct 5, 2010·Circulation Research·Glenn C RoweZolt Arany
May 10, 2011·General and Comparative Endocrinology·Junfeng SunHongfei Ji
Jun 17, 2011·The Journal of Sexual Medicine·Culley C Carson, Giuseppe Rosano
Apr 13, 2012·The Journal of Clinical Endocrinology and Metabolism·Molly M ShoresAlvin M Matsumoto
Sep 1, 2012·Annual Review of Genetics·David C Chan
Sep 1, 2012·Circulation Research·Vlad G Zaha, Lawrence H Young
Apr 4, 2013·The Journal of Endocrinology·Daniel M Kelly, T Hugh Jones
Jun 19, 2013·The Journal of Steroid Biochemistry and Molecular Biology·James R BellLea M D Delbridge
Oct 10, 2013·Journal of the American Heart Association·Marie-Hélène DisatnikDaria Mochly-Rosen

❮ Previous
Next ❯

Citations

Jul 7, 2018·Heart Failure Reviews·Vittorio Emanuele Bianchi
Sep 17, 2020·International Journal of Molecular Sciences·Ami KobayashiSatoshi Inoue
Mar 3, 2020·Experimental and Molecular Pathology·Haochang HuShuangshuang Wang
Mar 2, 2021·Molecular and Cellular Biochemistry·Ritesh Kumar SrivastavArshiya Shamim
Mar 2, 2021·Frontiers in Cell and Developmental Biology·Shannon LynchSamantha Giordano-Mooga
Mar 12, 2021·The Aging Male : the Official Journal of the International Society for the Study of the Aging Male·Sakthi Jothi Muthu, Prakash Seppan
Nov 24, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Cláudia FerreiraRita Nogueira-Ferreira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis