Tet-mediated imprinting erasure in H19 locus following reprogramming of spermatogonial stem cells to induced pluripotent stem cells.

Scientific Reports
P Bermejo-ÁlvarezB P Telugu

Abstract

Selective methylation of CpG islands at imprinting control regions (ICR) determines the monoparental expression of a subset of genes. Currently, it is unclear whether artificial reprogramming induced by the expression of Yamanaka factors disrupts these marks and whether cell type of origin affects the dynamics of reprogramming. In this study, spermatogonial stem cells (SSC) that harbor paternalized imprinting marks, and fibroblasts were reprogrammed to iPSC (SSCiPSC and fiPSC). The SSCiPSC were able to form teratomas and generated chimeras with a higher skin chimerism than those derived from fiPSC. RNA-seq revealed extensive reprogramming at the transcriptional level with 8124 genes differentially expressed between SSC and SSCiPSC and only 490 between SSCiPSC and fiPSC. Likewise, reprogramming of SSC affected 26 of 41 imprinting gene clusters known in the mouse genome. A closer look at H19 ICR revealed complete erasure in SSCiPSC in contrast to fiPSC. Imprinting erasure in SSCiPSC was maintained even after in vivo differentiation into teratomas. Reprogramming of SSC from Tet1 and Tet2 double knockout mice however lacked demethylation of H19 ICR. These results suggest that imprinting erasure during reprogramming depends on the e...Continue Reading

References

Feb 27, 1997·Nature·I WilmutK H Campbell
Jul 15, 1999·International Journal of Cancer. Journal International Du Cancer·R FukuzawaJ Hata
Aug 11, 2001·Science·A C Ferguson-Smith, M A Surani
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi KubotaRalph L Brinster
Dec 5, 2003·Mechanisms of Development·Lorraine E YoungRobert Feil
Nov 10, 2004·Genomics·Jing-Yu LiColum P Walsh
Dec 29, 2004·Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 18, 2005·Cancer Cell·Teresa M HolmRudolf Jaenisch
Jul 3, 2009·Cell Stem Cell·Kinarm KoHans R Schöler
Dec 17, 2009·Nature Methods·Bryce W CareyRudolf Jaenisch
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·P Bermejo-AlvarezA Gutierrez-Adan
Mar 18, 2010·Nature Methods·Nils GehlenborgAnne-Claude Gavin
Jun 24, 2010·Cell Stem Cell·Aviva Presser AidenBradley E Bernstein
Jul 21, 2010·Nature·K KimG Q Daley
Mar 30, 2012·Nature·Zachary D SmithAlexander Meissner
Dec 12, 2012·Molecular Cell·Stefanie SeisenbergerWolf Reik
Dec 18, 2012·The EMBO Journal·Saya KagiwadaMitinori Saitou
Dec 3, 2013·Nature·Shinpei YamaguchiYi Zhang

❮ Previous
Next ❯

Citations

Feb 18, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Sandra LaurentinoJörg Gromoll
May 9, 2018·Stem Cells and Development·Elena Gonzalez-Munoz, Jose B Cibelli
Jun 27, 2018·The Journal of Clinical Investigation·Isabel PuigHéctor G Palmer
Jun 3, 2020·ELife·Ismael Lamas-ToranzoPablo Bermejo-Álvarez
Feb 2, 2019·BMC Genomics·Alexander KanitzMihaela Zavolan
Oct 23, 2019·ELife·Ismael Lamas-ToranzoPablo Bermejo-Álvarez
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·I Lamas-ToranzoP Bermejo-Álvarez
Nov 17, 2020·Frontiers in Immunology·Bin WangGang Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE64856

Methods Mentioned

BETA
RNA-seq
PCR
transgenic
in vitro transcription
biopsy

Software Mentioned

Cufflinks
Cummerbund R
Tophat
DAVID Functional Annotation Tools
SigmaStat

Related Concepts

Related Feeds

Cardiovascular Disease & TET2

Cardiovascular diseases are the number one cause of deaths globally. Tet methylcytosine dioxygenase 2 (TET2)-mediated hematopoiesis has been implicated in accelerating heart failure. Here is the latest research on cardiovascular diseases and TET2.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.