PMID: 8968952Oct 1, 1996Paper

Tet repressor-tetracycline interaction

Journal of Protein Chemistry
P KaszyckiZ Wasylewski

Abstract

Previous studies [Wasylewski et al. (1996), J. Protein Chem. 15, 45-58] have shown that the W43 residue localized within the helix-turn-helix structure domain of Tet repressor can exist in the ground state in two conformational states. In this paper we investigate the fluorescence properties of W43 of TetR upon binding of tetracycline inducer and its chemical analogs such as anhydro- and epitetracycline. Binding of the drug inducer to the protein indicates that the W43 residue still exists in two conformational states; however, its environment changes drastically, as can be judged by the changes in fluorescence parameters. The FQRS (fluorescence-quenching-resolved spectra) method was used to decompose the total emission spectrum. The resolved spectra exhibit maxima of fluorescence at 346 and 332 nm and the component quenchable by KI (346 nm) is shifted 9 nm toward the blue side of the spectrum upon inducer binding. The observed shift does not result from the changes in the exposure of W43, since the bimolecular quenching rate constant remains the same and is equal to about 2.7 x 10(9) M-1 sec-1. The binding of tetracycline leads to drastic decrease of the W43 fluorescence intensity and increase of the tetracycline intensity as ...Continue Reading

References

Jan 1, 1992·Biophysical Chemistry·J R SmallE W Small
May 15, 1992·European Journal of Biochemistry·Z WasylewskiK Slizowska
Aug 25, 1989·Nucleic Acids Research·K Tovar, W Hillen
Mar 15, 1988·European Journal of Biochemistry·Z WasylewskiA Wasniowska
Jan 1, 1985·Annual Review of Biochemistry·J M Beechem, L Brand
Jan 1, 1994·Annual Review of Microbiology·W Hillen, C Berens
Dec 1, 1993·Biophysical Journal·Z Bajzer, F G Prendergast
Jul 1, 1995·Journal of Protein Chemistry·M WasylewskiZ Wasylewski
Nov 1, 1995·Journal of Protein Chemistry·Z Blicharska, Z Wasylewski
Jan 1, 1996·Journal of Protein Chemistry·Z WasylewskiM Drwiega

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Citations

Dec 8, 2011·Chemistry Central Journal·Michal Pellach, Shlomo Margel
Nov 19, 2003·European Journal of Biochemistry·Sylwia Kedracka-Krok, Zygmunt Wasylewski
Jul 18, 2006·Journal of Molecular Biology·Sean E Reichheld, Alan R Davidson
Sep 1, 2016·Scientific Reports·Jason G Lomnitz, Michael A Savageau
Nov 13, 2008·Journal of Agricultural and Food Chemistry·Nina E VirolainenMatti T Karp
Apr 13, 2006·Journal of the American Chemical Society·Frank R BeierleinTimothy Clark
Jan 19, 2005·Biochemistry·Sylwia Kedracka-KrokZygmunt Wasylewski

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