Tête à tête of tomato yellow leaf curl virus and tomato yellow leaf curl sardinia virus in single nuclei

Journal of Virology
Gabriel MorillaChristina Wege

Abstract

Since 1997 two distinct geminivirus species, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato yellow leaf curl virus (TYLCV), have caused a similar yellow leaf curl disease in tomato, coexisted in the fields of southern Spain, and very frequently doubly infected single plants. Tomatoes as well as experimental test plants (e.g., Nicotiana benthamiana) showed enhanced symptoms upon mixed infections under greenhouse conditions. Viral DNA accumulated to a similar extent in singly and doubly infected plants. In situ tissue hybridization showed TYLCSV and TYLCV DNAs to be confined to the phloem in both hosts, irrespective of whether they were inoculated individually or in combination. The number of infected nuclei in singly or doubly infected plants was determined by in situ hybridization of purified nuclei. The percentage of nuclei containing viral DNA (i.e., 1.4% in tomato or 6% in N. benthamiana) was the same in plants infected with either TYLCSV, TYLCV, or both. In situ hybridization of doubly infected plants, with probes that discriminate between both DNAs, revealed that at least one-fifth of infected nuclei harbored DNAs from both virus species. Such a high number of coinfected nuclei may explain why recombination bet...Continue Reading

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Citations

Jun 21, 2012·Molecular Plant-microbe Interactions : MPMI·Ana P LunaEduardo R Bejarano
Aug 13, 2011·Journal of Virology·T PaprotkaH Jeske
May 23, 2009·BMC Evolutionary Biology·Mart KrupovicDennis H Bamford
Mar 10, 2011·Virology Journal·Ilenia Rentería-CanettRafael F Rivera-Bustamante
Dec 3, 2014·Virus Research·Patricia WyantHolger Jeske
Mar 1, 2007·Molecular Plant Pathology·Andrew J MauleMargaret I Boulton
Dec 25, 2009·The Journal of General Virology·Julia B ErdmannHolger Jeske
Jun 9, 2012·Virus Research·Patricia Soares WyantChristina Wege
Oct 8, 2016·Virology·Kathrin DeuschleHolger Jeske
Sep 18, 2015·Journal of Virology·Karin WeigelHolger Jeske
Jul 14, 2010·Molecular Plant Pathology·Juan Antonio Díaz-PendónJesús Navas-Castillo
Feb 28, 2007·The Journal of General Virology·Diana Pohl, Christina Wege
Mar 4, 2006·Journal of Virological Methods·Daniela HaibleHolger Jeske

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