Tethering KSRP, a decay-promoting AU-rich element-binding protein, to mRNAs elicits mRNA decay.

Molecular and Cellular Biology
Chu-Fang ChouChing-Yi Chen

Abstract

Inherently unstable mRNAs contain AU-rich elements (AREs) in their 3' untranslated regions that act as mRNA stability determinants by interacting with ARE-binding proteins (ARE-BPs). We have destabilized two mRNAs by fusing sequence-specific RNA-binding proteins to KSRP, a decay-promoting ARE-BP, in a tethering assay. These results support a model that KSRP recruits mRNA decay machinery/factors to elicit decay. The ability of tethered KSRP to elicit mRNA decay depends on functions of known mRNA decay enzymes. By targeting the Rev response element of human immunodeficiency virus type 1 by using Rev-KSRP fusion protein, we degraded viral mRNA, resulting in a dramatic reduction of viral replication. These results provide a foundation for the development of novel therapeutic strategies to inhibit specific gene expression in patients with acquired or hereditary diseases.

References

Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Feb 24, 1997·The Journal of Cell Biology·V I BashkirovW D Heyer
Jan 19, 1999·Annual Review of Microbiology·V W Pollard, M H Malim
Aug 31, 2000·Annual Review of Biochemistry·M Tucker, R Parker
Mar 20, 2001·Gene·J Guhaniyogi, G Brewer
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·C J WiluszS W Peltz
Apr 6, 2001·Cellular and Molecular Life Sciences : CMLS·C M Brennan, J A Steitz
Aug 29, 2002·The EMBO Journal·Georg StoecklinChristoph Moroni
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zuoren WangMegerditch Kiledjian
Nov 21, 2002·Biochemical Society Transactions·P J Blackshear
Jan 10, 2003·Nucleic Acids Research·Tala BakheetKhalid S A Khabar
Apr 22, 2003·Journal of Cellular Physiology·Annamaria BevilacquaAngelo Nicolin
Aug 23, 2003·Trends in Biochemical Sciences·Bryan R Cullen
Aug 29, 2003·Molecular and Cellular Biology·Bedabrata SarkarRobert J Schneider
Oct 30, 2003·The Journal of Biological Chemistry·Naoyuki UchidaToshiaki Katada
Feb 21, 2004·Nucleic Acids Research·Ines RaineriChristoph Moroni
Apr 8, 2004·Virus Research·Nan Sook Lee, John J Rossi
Sep 15, 2004·Trends in Genetics : TIG·Carol J Wilusz, Jeffrey Wilusz
Nov 18, 2004·Molecular Cell·Leonie Unterholzner, Elisa Izaurralde
Feb 11, 2005·Gene Therapy·R Wolkowicz, G P Nolan
Nov 15, 2005·Nature Structural & Molecular Biology·Akio YamashitaAnn-Bin Shyu

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Citations

May 25, 2010·Cellular and Molecular Life Sciences : CMLS·Khalid S A Khabar
Mar 20, 2008·Nature Immunology·Paul Anderson
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Nicole L GarneauCarol J Wilusz
Mar 13, 2008·Nature Reviews. Molecular Cell Biology·Aaron C Goldstrohm, Marvin Wickens
Feb 10, 2009·Nature Structural & Molecular Biology·Irene Díaz-MorenoAndres Ramos
Jun 24, 2010·The Journal of Biological Chemistry·Daniella IshimaruEleanor K Spicer
Dec 2, 2008·Molecular Human Reproduction·Christine E BellAndrew J Watson
May 2, 2012·Nucleic Acids Research·David HollingworthAndres Ramos
Jun 15, 2007·Current Opinion in Nephrology and Hypertension·Tally Naveh-Many, Morris Nechama
Mar 21, 2007·Genes & Development·Tobias M Franks, Jens Lykke-Andersen
Jul 12, 2011·Eukaryotic Cell·Christopher J UtterVivian Bellofatto
Feb 19, 2008·Cancer Research·Jack ZhangG Tim Bowden
Apr 18, 2007·BMC Molecular Biology·Tina RuggieroRoberto Gherzi
Nov 12, 2010·RNA·Daniel L Kiss, Erik D Andrulis
Mar 29, 2008·RNA·Lara AjamianAndrew J Mouland
Sep 12, 2013·PloS One·Chu-Fang ChouChing-Yi Chen
Apr 16, 2008·The Journal of Cell Biology·Christopher von Roretz, Imed-Eddine Gallouzi
Jan 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ying-Ying WangShu-Yun Yang
Mar 29, 2012·Gene·Xiangyue Wu, Gary Brewer
Jun 7, 2011·Trends in Genetics : TIG·Alexa M Dickson, Jeffrey Wilusz
Oct 20, 2009·Molecular and Cellular Neurosciences·Silvia De Rubeis, Claudia Bagni
Jan 18, 2008·Biology of the Cell·Cecile Keryer-BibensH Beverley Osborne
Apr 5, 2013·Immunological Reviews·Pavel Ivanov, Paul Anderson
Sep 22, 2011·Wiley Interdisciplinary Reviews. RNA·Roberto GherziPaola Briata
Sep 22, 2011·Wiley Interdisciplinary Reviews. RNA·You Li, Megerditch Kiledjian
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Sharon H M LingHaiwei Song
Dec 10, 2009·Cellular Microbiology·Edith M C ChowJeremy Mogridge
Mar 19, 2013·Critical Reviews in Biochemistry and Molecular Biology·Anders VirtanenMikael Nissbeck
Apr 18, 2015·Nucleic Acids Research·Anna Kurzynska-KokorniakMarek Figlerowicz
Mar 4, 2014·Journal of Biological Engineering·Zhanar AbilHuimin Zhao
May 26, 2009·Biochimica Et Biophysica Acta·Wan-Cheol Kim, Chow H Lee

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