TetR family member psrA directly binds the Pseudomonas rpoS and psrA promoters.

Journal of Bacteriology
Milan KojicVittorio Venturi

Abstract

We have previously described a Pseudomonas gene, psrA, which enhances transcription of the rpoS sigma factor gene at stationary phase. We present molecular data which demonstrate that in Pseudomonas putida PsrA binds specifically to the rpoS and psrA promoters in DNA regions having similar palindromic sequences, C/GAAAC N(2-4) GTTTG/C, where N is any nucleotide. The position of the initiation of transcription was determined for both promoters, and PsrA binds from positions -59 to -35 in the rpoS promoter and from -18 to +20 in the psrA promoter with respect to the +1 transcription site. Expression studies with a psrA-lacZ transcriptional fusion in wild-type and psrA::Tn5 knockout mutants revealed that psrA was under additional control in response to growth phase. A model for the role of PsrA in the regulation of rpoS and psrA is presented.

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Citations

Jan 4, 2007·Current Microbiology·Yi-He GeYu-Quan Xu
Nov 13, 2012·Nucleic Acids Research·Deepak BalasubramanianKalai Mathee
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Thomas F C Chin-A-WoengGuido V Bloemberg
Sep 30, 2008·Journal of Bacteriology·Alexey E KazakovMikhail S Gelfand
Nov 4, 2005·Journal of Bacteriology·Karen PreiterBarbara N Kunkel
Jun 15, 2011·Trends in Microbiology·Elena B M BreidensteinRobert E W Hancock
Feb 14, 2006·FEMS Microbiology Reviews·Vittorio Venturi
May 19, 2005·FEMS Microbiology Letters·Milan KojicVittorio Venturi
May 6, 2020·Journal of Bacteriology·Ryan R ChaparianJulia C van Kessel
Dec 3, 2016·The ISME Journal·Peter A LindPaul B Rainey
Jan 8, 2021·RNA Biology·María-Dolores FerreiroMaría-Trinidad Gallegos
May 9, 2021·Microbiological Research·Claudia Velázquez-SánchezDaniel Segura
May 22, 2021·Molecular Microbiology·Christopher I GrahamAnn Karen C Brassinga

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