Tetracapsuloides bryosalmonae infection affects the expression of genes involved in cellular signal transduction and iron metabolism in the kidney of the brown trout Salmo trutta

Parasitology Research
Gokhlesh KumarMansour El-Matbouli

Abstract

Tetracapsuloides bryosalmonae is an enigmatic endoparasite which causes proliferative kidney disease in various species of salmonids in Europe and North America. The life cycle of the European strain of T. bryosalmonae generally completes in an invertebrate host freshwater bryozoan and vertebrate host brown trout (Salmo trutta) Linnaeus, 1758. Little is known about the gene expression in the kidney of brown trout during the developmental stages of T. bryosalmonae. In the present study, quantitative real-time PCR was applied to quantify the target genes of interest in the kidney of brown trout at different time points of T. bryosalmonae development. PCR primers specific for target genes were designed and optimized, and their gene expression levels were quantified in the cDNA kidney samples using SYBR Green Supermix. Expression of Rab GDP dissociation inhibitor beta, integral membrane protein 2B, NADH dehydrogenase 1 beta subcomplex subunit 6, and 26S protease regulatory subunit S10B were upregulated significantly in infected brown trout, while the expression of the ferritin M middle subunit was downregulated significantly. These results suggest that host genes involved in cellular signal transduction, proteasomal activities, inc...Continue Reading

References

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Citations

Oct 9, 2015·Environmental Science and Pollution Research International·Subhodeep SarkerA Sarkar
May 30, 2020·International Journal of Molecular Sciences·Arun SudhagarGokhlesh Kumar
Jun 9, 2018·BMC Veterinary Research·Michael GotesmanMansour El-Matbouli

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Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

SIDAK

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