PMID: 9441722Jan 27, 1998Paper

Tetracycline-induced steatosis in primary canine hepatocyte cultures

Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology
D E Amacher, B A Martin

Abstract

Primary hepatocyte cultures prepared from male beagle dog liver were used to determine susceptibility of the canine liver to tetracycline-induced steatosis. The effects of the drug on mitochondrial lipid metabolism and intracellular triglyceride accumulation were monitored at the same time that steatosis was detected by light microscopy and quantitated using lipid-specific stains. Exposure of primary canine hepatocyte cultures to tetracycline for 24-48 h resulted in concentration-dependent, significant increases in the Oil Red O-stained lipid inclusions. Microscopic examination of the total stained areas suggested that increases over control levels were due primarily to the increase in the size of the lipid inclusions rather than in the number. Biochemical analyses for triglyceride content and histological staining with Nile red, another neutral lipid-specific dye, confirmed a specific increase in intracellular triglyceride following a 24-h exposure to noncytotoxic levels of tetracycline beta-oxidation studies based on the oxidation of [14C]palmitic acid or [14C]palmitoyl carnitine demonstrated a concentration-dependent inhibition of mitochondrial but not peroxisomal beta-oxidation in hepatocytes after a 24-h exposure to tetrac...Continue Reading

Citations

Nov 28, 2013·Experimental Cell Research·Ansgar BrüningIoannis Mylonas
Feb 24, 2006·Antimicrobial Agents and Chemotherapy·Benjamin L MakepeaceAlexander J Trees
May 26, 2009·Toxicology and Applied Pharmacology·Chong ShenAugustinus Bader
Dec 26, 2006·Chemico-biological Interactions·María José Gómez-LechónJosé-Enrique O'Connor
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