Tetracycline-regulated transactivators driven by the involucrin promoter to achieve epidermal conditional gene expression

The Journal of Investigative Dermatology
Jean JaubertJulia A Segre

Abstract

To achieve conditional gene expression in the differentiated layers of the epidermis, we generated transgenic mice with the tetracycline-regulated transactivator proteins, tTA (tetracycline transactivator) and rtTA (reverse tetracycline transactivator), expressed from the human involucrin promoter. Interaction with tetracycline turns off or turns on the tTA and rtTA molecules, respectively, allowing for regulation of downstream target genes during development and postnatally. These transactivator lines were crossed with reporter mice driving LacZ expression from a tetracycline response element to analyze the specificity and levels of target gene expression. Quantitative beta-galactosidase experiments demonstrate a 30-fold induction, specific to epithelial tissues. Immunohistochemistry results illustrate that the beta-galactosidase staining follows that of endogenous involucrin expression. Induction initiates at embryonic day 14.5 with expression over the entire epidermal surface by E16.5. Together with other driver lines, expressing tetracycline transactivators in the mitotically active layers of the epidermis, these mice will allow investigators to specifically modulate expression of target genes to specific stages of epiderma...Continue Reading

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Citations

Dec 24, 2010·American Journal of Physiology. Cell Physiology·Ping HuangAndrew J Paterson
Apr 22, 2006·The Journal of Clinical Investigation·Ali R DjalilianJulia A Segre
Jul 23, 2014·The Journal of Investigative Dermatology·Ellen A RorkeRichard L Eckert
Aug 14, 2009·The Journal of Investigative Dermatology·Jaana MannikSoosan Ghazizadeh
Nov 16, 2012·The Journal of Investigative Dermatology·Andrew J GundersonAdam B Glick
Jun 14, 2014·The Journal of Investigative Dermatology·Christian GünschmannLukas Scharfenberger
Oct 30, 2007·Journal of Dermatological Science·Priyadharsini Nagarajan, Satrajit Sinha
Apr 3, 2012·Genesis : the Journal of Genetics and Development·Marlon R Schneider
Mar 5, 2004·Biochimica Et Biophysica Acta·Brian M PolsterJ Marie Hardwick
Mar 14, 2020·Science·Felipe Garcia QuirozElaine Fuchs
Oct 11, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Douglas F PinheiroIris K Gratz

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