PMID: 2123338Aug 1, 1990Paper

Tetraethylammonium ion sensitivity of a 35-pS CA2(+)-activated K+ channel in GH3 cells that is activated by thyrotropin-releasing hormone

Pflügers Archiv : European journal of physiology
D G Lang, A K Ritchie

Abstract

Single Ca2(+)-activated K+ channels were studied in membrane patches from the GH3 anterior pituitary cell line. We have previously demonstrated the coexistence of large-conductance and small-conductance (280 pS and 11 pS in symmetrical 150 mM K+, respectively) Ca2(+)-activated K+ channels in this cell line (Lang and Ritchie 1987). Here we report the existence of a third type of Ca2(+)-activated K+ channel that has a conductance of about 35 pS under similar conditions. In excised inside-out patches, this channel can be activated by elevations of the internal free Ca2+ concentration, and the open probability increases as the membrane potential is made more positive. In excised patches, the sensitivity of this 35-pS channel to internal Ca2+ is low; at positive membrane potentials, this channel requires Ca2+ concentrations greater than 10 microM for activation. However, 35-pS channels have a much higher sensitivity to Ca2+ in the first minute after excision (activated by 1 microM Ca2+ at -50 mV). Therefore, it is possible that the Ca2+ sensitivity of this channel is stabilized by intracellular factors. In cell-attached patches, this intermediate conductance channel can be activated (at negative membrane potentials) by thyrotropin-r...Continue Reading

References

Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M Dubinsky, G S Oxford
Jan 1, 1986·The Journal of General Physiology·D R Matteson, C M Armstrong
Jul 1, 1987·The Journal of General Physiology·A Hermann, C Erxleben
Dec 1, 1987·Pflügers Archiv : European journal of physiology·D G Lang, A K Ritchie
Mar 1, 1989·The Journal of Physiology·G S Oxford, P K Wagoner
Mar 1, 1981·Pflügers Archiv : European journal of physiology·H D LuxA Marty
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Mar 1, 1984·The Journal of General Physiology·J M Dubinsky, G S Oxford
Mar 1, 1984·The Journal of General Physiology·D R Matteson, C M Armstrong

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Citations

Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·P Mollard, W Schlegel
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
May 9, 2012·Journal of Neuroendocrinology·S S StojilkovicC A Stratakis
Jul 24, 2010·IEEE Transactions on Nanobioscience·Glen R KirkhamSarah Harriet Cartmell
Sep 1, 2015·Biochemical and Biophysical Research Communications·Alsu N MustafinaGuzel F Sitdikova
Apr 10, 2015·Journal of Cardiovascular Pharmacology·Lasse SkibsbyeThomas Jespersen
Nov 18, 2000·American Journal of Physiology. Cell Physiology·A CzarneckiL Bresson-Bepoldin

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