Tetrahedral DNA Nanomaterial Regulates the Biological Behaviors of Adipose-Derived Stem Cells via DNA Methylation on Dlg3

ACS Applied Materials & Interfaces
Shiyu LinYunfeng Lin

Abstract

As a simple and classical three-dimensional shape, tetrahedral DNA nanostructures (TDNs) can provide robust properties for better stability and can serve as a versatile platform for biosensing and drug delivery. More in-depth, its safety should be assessed by sensitive detection methods. However, the effect of TDNs at the epigenetic level has not received much attention. Here, DNA methylation alteration in adipose-derived stem cells (ASCs) after exposure to TDNs was comprehensively evaluated. The results from reduced representation bisulfite sequencing, bisulfite-specific polymerase chain reaction, and further gene function analysis revealed that TDNs induced a few differentially methylated regions where negatively correlated gene expressions occur. Moreover, TDNs facilitated ASC proliferation and attenuated apoptosis via DNA hypermethylation of the Dlg3 gene promotor. This study may help pave the way for potential applications with the nanosafety of TDNs and offer deep insights into the proliferation promotion effect and antiapoptosis ability of TDNs.

References

Jul 18, 1997·Science·B AntonssonJ C Martinou
Jan 10, 2002·Nature·Hao YanNadrian C Seeman
Jan 10, 2002·Genes & Development·Adrian Bird
Jan 12, 2002·Plastic and Reconstructive Surgery·Hiroshi MizunoMarc H Hedrick
May 10, 2003·Nature Cell Biology·Nathalie SansRobert J Wenthold
Jul 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chunhai FanAlan J Heeger
Mar 5, 2004·Biochimica Et Biophysica Acta·Martin R Sprick, Henning Walczak
Jun 15, 2005·Annual Review of Biochemistry·Mary Grace Goll, Timothy H Bestor
May 8, 2008·Journal of the American Chemical Society·Gang LiuChunhai Fan
Nov 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jamie I FletcherJerry M Adams
Oct 16, 2009·The New England Journal of Medicine·Richard S HotchkissPaul E Swanson
Nov 16, 2010·Developmental Cell·María Berdasco, Manel Esteller
Oct 22, 2011·Cell Death and Differentiation·G DewsonR M Kluck
Nov 8, 2011·Nature Nanotechnology·Andre V PinheiroHao Yan
Feb 22, 2012·Science·Shawn M DouglasGeorge M Church
Jun 5, 2012·Nature Nanotechnology·Hyukjin LeeDaniel G Anderson
Feb 6, 2013·Chemical Communications : Chem Comm·Kyoung-Ran KimDae-Ro Ahn
Apr 3, 2013·Cold Spring Harbor Perspectives in Biology·David R McIlwainTak W Mak
Oct 11, 2013·Cell Cycle·Alessandra AlteriMilena Grossi
Jan 2, 2014·Accounts of Chemical Research·Hao PeiChunhai Fan
Apr 14, 2015·Molecular Cell·Xinjian LiuChuan-Yuan Li
May 6, 2015·Nanotoxicology·Xiaoyan LuPhilip Demokritou
Apr 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Emily A SaundersonJohannes M H M Reul
Apr 19, 2018·ACS Applied Materials & Interfaces·Kai XiaYing Zhu
Jun 28, 2018·Cell Proliferation·Gelina S KopeinaBoris Zhivotovsky
Jul 11, 2018·ACS Applied Materials & Interfaces·Xiaoxing ChenZhe Li
Nov 1, 2017·Journal of Biomedical Nanotechnology·Chang LiuHaiyun Song

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Citations

Jul 18, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Menglei WangLi Li
Mar 30, 2019·ACS Applied Materials & Interfaces·Yuxi ZhanYunfeng Lin
Aug 7, 2019·ACS Applied Materials & Interfaces·Mei ZhangXiaoxiao Cai
Dec 19, 2018·ACS Applied Materials & Interfaces·Chenchen MaoYunfeng Lin
Sep 26, 2020·ACS Applied Materials & Interfaces·Tao ZhangYunfeng Lin
Mar 28, 2020·Current Stem Cell Research & Therapy·Weitong CuiTianxu Zhang
Apr 24, 2020·Current Stem Cell Research & Therapy·Xiaolin ZhangXiaoxiao Cai

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