Tetrahydrobiopterin augments arginine transport in rat cardiac myocytes through modulation of CAT-2 mRNA

The Journal of Laboratory and Clinical Medicine
Idit F SchwartzAdrian Iaina

Abstract

Tetrahydrobiopterin (BH4) has been shown to be required for dimerization and acquisition of nitric oxide (NO) generating capacity by nitric oxide synthase (NOS). In the present study we have investigated the hypothesis that BH4 may affect NOS activity through a novel mechanism-namely, modulating arginine transport in rat cardiac myocytes. Cardiac myocytes have been previously shown to express cationic amino acid transport proteins (y+ system) CAT-1 and CAT-2. Increasing extracellular BH4 concentrations up to 0.5 mmol/L augments arginine transport in 1 mmol/L arginine media (no BH4, 558 +/- 42 fmol arginine/microg protein/min; 0.1 mmol/L BH4, 580 +/- 11 fmol arginine/microg protein/min; 0.5 mmol/L BH4, 944 +/- 71* fmol arginine/microg protein/min; 1.0 mmol/L BH4, 983+/-84* fmol arginine/microg protein/min, n = 4; *: P <.05 vs no BH4). Treating the cells with lipopolysaccharide (LPS) (10 microg/mL) significantly augmented arginine transport only in the presence of BH4 (no BH4, 600 +/- 33 fmol arginine/microg protein/min; 0.1 mmol/L BH4, 691 +/- 29*dagger fmol arginine/microg protein/min; 0.5 mmol/L BH4, 1123 +/- 32*dagger fmol arginine/microg protein/min; 1.0 mmol/L BH4, 1296 +/- 42*dagger fmol arginine/microg protein/min, n = 4;...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J GiovanelliS Kaufman
Jan 1, 1990·The Journal of Clinical Investigation·D L GrangerD T Durack
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R IyengarM A Marletta
Jul 1, 1984·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B Bartholomew
Jan 1, 1994·Annual Review of Biochemistry·D S Bredt, S H Snyder
Jan 1, 1995·Annual Review of Physiology·S S Gross, M S Wolin
May 1, 1995·Naunyn-Schmiedeberg's Archives of Pharmacology·B Mayer, E R Werner
Mar 28, 1997·The Journal of Biological Chemistry·H HosokawaT Masaki
Aug 8, 1997·The Journal of Biological Chemistry·S L HyattM Hatzoglou
Jun 17, 1998·The Journal of Biological Chemistry·B NicholsonC L MacLeod

❮ Previous
Next ❯

Citations

Mar 17, 2007·The Journal of Physiology·Carmelle V Remillard, Jason X-J Yuan
Dec 16, 2004·American Journal of Physiology. Heart and Circulatory Physiology·S C SchäferH-A Lehr
Dec 26, 2001·Current Opinion in Nephrology and Hypertension·Ellen I Closs
Dec 12, 2002·American Journal of Physiology. Renal Physiology·Doron SchwartzAdrian Iaina
May 11, 2004·Clinical and Experimental Hypertension : CHE·Y ZhangJudith A Whitworth
Jul 2, 2003·Nutrition·Undurti N Das

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.