Tetrahydrobiopterin Inhibits PDGF-stimulated Migration and Proliferation in Rat Aortic Smooth Muscle Cells via the Nitric Oxide Synthase-independent Pathway.

The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Xiaowen JiangKyung-Jong Won

Abstract

Tetrahydrobiopterin (BH4), an essential cofactor for nitric oxide synthase (NOS) activity, is known to play important roles in modulating both NO and superoxide production during vascular diseases such as atherosclerosis. However, the role of BH4 in functions of vascular smooth muscle cells is not fully known. In this study, we tested the effects of BH4 and dihydrobiopterin (BH2), a BH4 precursor, on migration and proliferation in response to platelet-derived growth factor-BB (PDGF-BB) in rat aortic smooth muscle cells (RASMCs). Cell migration and proliferation were measured using a Boyden chamber and a 5-bromo-2'-deoxyuridine incorporation assay, respectively, and these results were confirmed with an ex vivo aortic sprout assay. Cell viability was examined by 2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide assays. BH4 and BH2 decreased PDGF-BB-induced cell migration and proliferation in a dose-dependent manner. The inhibition of cell migration and proliferation by BH4 and BH2 was not affected by pretreatment with N(G)-nitro-L-arginine methyl ester, a NOS inhibitor. Moreover, the sprout outgrowth formation of aortic rings induced by PDGF-BB was inhibited by BH4 and BH2. Cell viability was not inhibited ...Continue Reading

References

Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C A NicholD S Duch
May 1, 1995·Naunyn-Schmiedeberg's Archives of Pharmacology·B Mayer, E R Werner
Dec 31, 1997·The European Journal of Neuroscience·P Z AnastasiadisR A Levine
Aug 10, 1999·Japanese Journal of Pharmacology·S ShimizuT Yamamoto
Oct 3, 1999·Physiological Reviews·C H Heldin, B Westermark
Jan 2, 2001·The Journal of Biological Chemistry·P Z AnastasiadisR A Levine
Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Z S Katusic
Dec 6, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Nicholas J Alp, Keith M Channon
Jun 23, 2004·Cytokine & Growth Factor Reviews·Elaine W Raines
Mar 19, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Chao-Hung WangSubodh Verma
Dec 6, 2005·Journal of Biomedical Science·Giovanni Maria PudduPaolo Puddu
Sep 2, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·An L Moens, David A Kass
Oct 21, 2006·Vascular Pharmacology·Gunay Yetik-Anacak, John D Catravas
Sep 20, 2007·Journal of Cardiovascular Pharmacology·An L Moens, David A Kass

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Citations

Dec 30, 2017·Applied Microbiology and Biotechnology·Thandeeswaran MurugesanAngayarkanni Jayaraman

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