Tetrahydrobiopterin Plays a Functionally Significant Role in Lipogenesis in the Oleaginous Fungus Mortierella alpina

Frontiers in Microbiology
Hongchao WangWei Chen

Abstract

Tetrahydrobiopterin (BH4) is well-known as a cofactor of phenylalanine hydroxylase (PAH) and nitric oxide synthase (NOS), but its exact role in lipogenesis is unclear. In this study, the GTP cyclohydrolase I (GTPCH) gene was overexpressed to investigate the role of BH4 in lipogenesis in oleaginous fungus Mortierella alpina. Transcriptome data analysis reveal that GTPCH expression was upregulated when nitrogen was exhausted, resulting in lipid accumulation. Significant changes were also found in the fatty acid profile of M. alpina grown on medium that contained a GTPCH inhibitor relative to that of M. alpina grown on medium that lacked the inhibitor. GTPCH overexpression in M. alpina (the MA-GTPCH strain) led to a sevenfold increase in BH4 levels and enhanced cell fatty acid synthesis and poly-unsaturation. Increased levels of nicotinamide adenine dinucleotide phosphate (NADPH) and upregulated expression of NADPH-producing genes in response to enhanced BH4 levels were also observed, which indicate a novel aspect of the NADPH regulatory mechanism. Increased BH4 levels also enhanced phenylalanine hydroxylation and nitric oxide synthesis, and the addition of an NOS or a PAH inhibitor in the MA-GTPCH and control strain cultures decr...Continue Reading

References

Jan 1, 1970·Annual Review of Microbiology·H S Forrest, C Van Baalen
Jan 1, 1995·Journal of Inherited Metabolic Disease·M GiovanniniE Riva
Jan 1, 1993·Annual Review of Nutrition·S Kaufman
Jun 27, 1998·The Journal of Biological Chemistry·F FusettiR C Stevens
Jul 21, 1998·The Journal of Biological Chemistry·L V MichaelsonA K Stobart
Nov 7, 1996·Chemical Reviews·T. Joseph Kappock, John P. Caradonna
Nov 18, 1970·FEBS Letters·S GrisoliaD Diederich
Nov 13, 2004·Applied Microbiology and Biotechnology·Eiji SakuradaniSakayu Shimizu
Mar 16, 2007·Journal of the American Society for Mass Spectrometry·Wenyun LuJoshua D Rabinowitz
Mar 23, 2007·Genes, Brain, and Behavior·W S NeckameyerS F Goodwin
Jan 4, 2008·American Journal of Physiology. Renal Physiology·Wei WangRobert Schrier
Jan 1, 1967·Annual Review of Biochemistry·S Kaufman
Mar 7, 2008·Antioxidants & Redox Signaling·Eberhard SchulzThomas Münzel
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Apr 2, 2009·American Journal of Physiology. Endocrinology and Metabolism·Lu TieAlex F Chen
Oct 12, 2012·Applied Microbiology and Biotechnology·Rosa Amarilis Rodríguez-FrómetaVictoriano Garre
Jan 1, 2013·G3 : Genes - Genomes - Genetics·Teresa Z Rzezniczak, Thomas J S Merritt
May 2, 2013·Critical Reviews in Biotechnology·Xiao-Jun JiPing-Kai Ouyang
Feb 18, 2014·Applied and Environmental Microbiology·Guangfei HaoYong Q Chen
May 9, 2014·Nature·Jing FanJoshua D Rabinowitz
Jul 8, 2014·Biochimica Et Biophysica Acta·Ju-Yi HsiehHui-Chih Hung
Feb 10, 2018·Applied Microbiology and Biotechnology·Marianna DourouGeorge Aggelis

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRR1638095

Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

Cufflinks

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.