Tetrahydrobiopterin prevents chronic ischemia-related lower urinary tract dysfunction through the maintenance of nitric oxide bioavailability.

Scientific Reports
Hidenori AkaihataYoshiyuki Kojima

Abstract

This study aimed to investigate the influence of chronic ischemia on nitric oxide biosynthesis in the bladder and the effect of administering tetrahydrobiopterin (BH4), a cofactor for endothelial nitric oxide synthase (eNOS), on chronic ischemia-related lower urinary tract dysfunction (LUTD). This study divided male Sprague-Dawley rats into Control, chronic bladder ischemia (CBI) and CBI with oral BH4 supplementation (CBI/BH4) groups. In the CBI group, bladder capacity and bladder muscle strip contractility were significantly lower, and arterial wall was significantly thicker than in Controls. Significant improvements were seen in bladder capacity, muscle strip contractility and arterial wall thickening in the CBI/BH4 group as compared with the CBI group. Western blot analysis of bladder showed expressions of eNOS (p = 0.043), HIF-1α (p < 0.01) and dihydrofolate reductase (DHFR) (p < 0.01), which could regenerate BH4, were significantly higher in the CBI group than in Controls. In the CBI/BH4 group, HIF-1α (p = 0.012) and DHFR expressions (p = 0.018) were significantly decreased compared with the CBI group. Our results suggest that chronic ischemia increases eNOS and DHFR in the bladder to prevent atherosclerosis progression. H...Continue Reading

References

Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C R WhiteB A Freeman
Apr 29, 1998·The Journal of Clinical Investigation·F CosentinoT F Lüscher
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Vásquez-VivarK A Pritchard
Sep 10, 1999·The Journal of Biological Chemistry·J Vásquez-VivarB Kalyanaraman
Oct 28, 1999·Cardiovascular Research·F Cosentino, T F Lüscher
Aug 7, 2002·The Journal of Clinical Investigation·Masanori OzakiMitsuhiro Yokoyama
Mar 8, 2006·European Urology·Anton PonholzerStephan Madersbacher
Apr 6, 2006·Clinical Medicine & Research·Zhihong Yang, Xiu-Fen Ming
Feb 3, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Yoshiyuki HattoriKikuo Kasai
Jan 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Mark J CrabtreeSteven S Gross
Sep 1, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Jianzhong AnYang Shi
Sep 10, 2011·Neurourology and Urodynamics·Masanori NomiyaNorio Takahashi
Sep 23, 2011·Molecular Genetics and Metabolism·Nenad BlauUta Lichter-Konecki
Jan 1, 2010·Integrated Blood Pressure Control·Alberto Francisco Rubio-GuerraBruno Alfonso Escalante-Acosta
Nov 29, 2011·European Journal of Pharmacology·Christian MoroRuss Chess-Williams
Sep 18, 2012·The Journal of Urology·Masanori NomiyaKarl-Erik Andersson
Oct 4, 2014·The Journal of Urology·Hidenori AkaihataYoshiyuki Kojima

❮ Previous
Next ❯

Methods Mentioned

BETA
urine collection
pharmacotherapy
electrophoresis

Software Mentioned

cellSens Dimension
GraphPad Prism
MacLab
SPSS Statistics

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.