Tetrahydrobiopterin reverses the impairment of acetylcholine-induced vasodilatation in diabetic ocular microvasculature

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
P K YuE N Su

Abstract

The purpose of this study is to test whether tetrahydrobiopterin, an essential cofactor in nitric oxide synthesis, can reverse endothelium dysfunction in diabetic ocular circulation. Using the streptozotocin-induced diabetic rat model and the isolated perfusion eye technique, the response to the acetylcholine (an endothelium-dependent vasodilator mediated by stimulated nitric oxide release) induced vasodilatation of the diabetic ocular vasculature before and after tetrahydrobiopterin administration was compared. Age matched normal rats were used for reference response. Six streptozotocin-induced diabetic rats and eleven control rats at 21.5 +/- 0.2 weeks and 21.2 +/- 2.1 weeks postinduction, respectively, were used. The dose response curve from the diabetic eyes was found to be significantly different from that of the control eyes (p < 0.001) with significantly reduced responses to 10(-4)M acetylcholine. After 30 min of administration of tetrahydrobiopterin to the diabetic eyes, however, the acetylcholine-induced vasodilatation response was significantly (p < 0.001) increased compared with the response prior to tetrahydrobiopterin administration. The vasodilatory response in the diabetic eyes after tetrahydrobiopterin administr...Continue Reading

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Citations

Mar 11, 2004·Current Hypertension Reports·Dierk H Endemann, Ernesto L Schiffrin
Jul 2, 2003·Nutrition·Undurti N Das
Feb 27, 2003·Progress in Retinal and Eye Research·Dao-Yi YuPaula K Yu
Nov 2, 2006·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Yoshiko KanekoKunio Ishii
Mar 4, 2014·Progress in Retinal and Eye Research·Dao-Yi YuEr-Ning Su
Sep 10, 2009·Brain Research Bulletin·Ruth B CaldwellR William Caldwell
Sep 19, 2006·Experimental Eye Research·Toshiaki GosekiKimiya Shimizu
Jul 19, 2008·Free Radical Biology & Medicine·Keiichi KomeimaPeter A Campochiaro
Aug 21, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Katherine A BlackwellZvonimir S Katusic
Aug 12, 2020·Visual Neuroscience·Cyril G EleftheriouBotir T Sagdullaev
Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Z S Katusic

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