Tetrahydrobiopterin Role in human umbilical vein endothelial dysfunction in maternal supraphysiological hypercholesterolemia

Biochimica Et Biophysica Acta
Andrea LeivaLuis Sobrevia

Abstract

Maternal physiological hypercholesterolemia (MPH) allows a proper foetal development; however, maternal supraphysiological hypercholesterolemia (MSPH) associates with foetal endothelial dysfunction and early development of atherosclerosis. MSPH courses with reduced endothelium-dependent dilation of the human umbilical vein due to reduced endothelial nitric oxide synthase activity compared with MPH. Whether MSPH modifies the availability of the nitric oxide synthase cofactor tetrahydrobiopterin is unknown. We investigated whether MSPH-associated lower umbilical vein vascular reactivity results from reduced bioavailability of tetrahydrobiopterin. Total cholesterol <7.2mmol/L was considered as maternal physiological hypercholesterolemia (n=72 women) and ≥7.2mmol/L as MSPH (n=35 women). Umbilical veins rings were used for vascular reactivity assays (wire myography), and primary cultures of human umbilical vein endothelial cells (HUVECs) to measure nitric oxide synthase, GTP cyclohydrolase 1, and dihydrofolate reductase expression and activity, as well as tetrahydrobiopterin content. MSPH reduced the umbilical vein rings relaxation caused by calcitonine gene-related peptide, a phenomenon partially improved by incubation with sepiapt...Continue Reading

References

Jan 1, 1991·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·E R WernerH Wachter
Mar 1, 1985·The Journal of Clinical Endocrinology and Metabolism·R H KnoppJ J Albers
Jan 1, 1997·The Journal of Clinical Investigation·E StroesT Rabelink
Aug 4, 2004·European Journal of Nuclear Medicine and Molecular Imaging·Christophe A WyssPhilipp A Kaufmann
Dec 21, 2007·Molecular Reproduction and Development·Charles Marseille-TremblayJulie Lafond
Feb 24, 2009·Reproductive Sciences·Ahmet Basaran
May 10, 2011·Nitric Oxide : Biology and Chemistry·Mark J Crabtree, Keith M Channon
Nov 30, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lacy M AlexanderW Larry Kenney
Dec 25, 2012·Current Vascular Pharmacology·Keith M Channon
Feb 12, 2013·Current Opinion in Pharmacology·Huige Li, Ulrich Förstermann
Apr 24, 2014·Diabetes Care·Helen L BarrettLeonie K Callaway
Aug 22, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Hanrui ZhangCatherine Martel
Apr 15, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·F PardoL Sobrevia

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