Abstract
We have previously shown that actin from Tetrahymena pyriformis has a very divergent primary structure (Hirono, M., Endoh, H., Okada, N., Numata, O., & Watanabe, Y. (1987) J. Mol. Biol. 194, 181-192) and that though it shares essential properties with skeletal muscle actin, it does not interact at all with phalloidin or DNase I (Hirono, M., Kumagai, Y., Numata, O., & Watanabe, Y. (1989) Proc. Natl. Acad. Sci. U.S. 86, 75-79). In this study, we investigated the copolymerization of this actin with skeletal muscle actin by direct observation of the heteropolymers formed from the two actins by means of electron microscopy. We also examined the binding of actin-binding proteins from skeletal muscle or smooth muscle to Tetrahymena actin by means of a cosedimentation assay. The results show that (i) Tetrahymena actin copolymerizes with skeletal muscle actin and that (ii) muscle myosin subfragment 1 binds to it in the absence of ATP, like skeletal muscle actin. However, it was also shown that (iii) muscle alpha-actinin hardly binds to Tetrahymena actin and that (iv) muscle tropomyosin does not bind to it at all. The results show that Tetrahymena actin has both properties similar and dissimilar to those of skeletal muscle actin.
Citations
Dec 2, 2005·Molecular Biology of the Cell·Nivedita SahooL David Sibley
Apr 1, 1995·Journal of Muscle Research and Cell Motility·M YamaguchiT Oba
Mar 1, 1994·Cancer Metastasis Reviews·S H Lo, L B Chen
Feb 1, 1995·Zoological Science·O NumataY Watanabe
Mar 6, 1995·FEBS Letters·I MurgiaP Morandini
Apr 25, 1994·FEBS Letters·E MutoR Kamiya
Jun 1, 1996·Zoological Science·Y KurasawaO Numata
Jan 1, 1992·Cell Motility and the Cytoskeleton·M L CanoS H Zigmond
Jan 1, 1990·Annals of the New York Academy of Sciences·Y WatanabeT Yasuda
May 12, 2004·The Journal of Eukaryotic Microbiology·Oanh T P KimTerue Harumoto
Aug 31, 2000·Biochemical and Biophysical Research Communications·K SaekiT Wakabayashi
Mar 15, 1991·Biochemical and Biophysical Research Communications·M EdamatsuY Watanabe
Jul 31, 1990·Biochemical and Biophysical Research Communications·M EdamatsuY Watanabe
Feb 24, 2000·Biochemistry·K Saeki, T Wakabayashi
Nov 19, 1996·Biochemistry·K SaekiT Wakabayashi
Oct 30, 1992·Science·K F Wertman, D G Drubin