Tetramer-dimer equilibrium of oxyhemoglobin mutants determined from auto-oxidation rates

Protein Science : a Publication of the Protein Society
Nathalie GriffonMichael C Marden

Abstract

One of the main difficulties with blood substitutes based on hemoglobin (Hb) solutions is the auto-oxidation of the hemes, a problem aggravated by the dimerization of Hb tetramers. We have employed a method to study the oxyHb tetramer-dimer equilibrium based on the rate of auto-oxidation as a function of protein concentration. The 16-fold difference in dimer and tetramer auto-oxidation rates (in 20 mM phosphate buffer at pH 7.0, 37 degrees C) was exploited to determine the fraction dimer. The results show a transition of the auto-oxidation rate from low to high protein concentrations, allowing the determination of the tetramer-dimer dissociation coefficient K4,2 = [Dimer] 2/[Tetramer]. A 14-fold increase in K4,2 was observed for addition of 10 mM of the allosteric effector inositol hexaphosphate (IHP). Recombinant hemoglobins (rHb) were genetically engineered to obtain Hb with a lower oxygen affinity than native Hb (Hb A). The rHb alpha2beta2 [(C7) F41Y/(G4) N102Y] shows a fivefold increase in K4,2 at pH 7.0, 37 degrees C. An atmosphere of pure oxygen is necessary in this case to insure fully oxygenated Hb. When this condition is satisfied, this method provides an efficient technique to characterize both the tetramer-dimer equi...Continue Reading

References

Nov 14, 1978·Biochemical and Biophysical Research Communications·A TsapisJ Rosa
Jan 25, 1977·Biochimica Et Biophysica Acta·K Imai, H Yonetani
Jul 15, 1976·The New England Journal of Medicine·R L NagelM B Harris
Jan 1, 1992·Protein Science : a Publication of the Protein Society·M T BihoreauC Poyart
Jan 3, 1992·Science·G K AckersM A Daugherty
Oct 1, 1991·Biophysical Journal·M C MardenC Poyart
May 1, 1988·Journal of Biochemical and Biophysical Methods·M A Khaleque, C A Sawicki
Sep 15, 1988·European Journal of Biochemistry·H Schönert, B Stoll
May 11, 1971·Biochemistry·H HamaguchiH Nakajima
Jul 1, 1972·Archives of Biochemistry and Biophysics·S StefaniniE Antonini
Nov 28, 1970·Journal of Molecular Biology·J W Goers, V N Schumaker
Jan 1, 1969·The Journal of Clinical Investigation·H F Bunn
Apr 28, 1967·Journal of Molecular Biology·M A Rosemeyer, E R Huehns
Dec 15, 1980·Journal of Molecular Biology·V S SharmaE H Danish
Aug 1, 1981·Biophysical Journal·C A Sawicki, Q H Gibson
Jun 9, 1995·The Journal of Biological Chemistry·R E Benesch, S Kwong
Jan 1, 1994·Methods in Enzymology·V W Macdonald
Jan 1, 1993·The Japanese Journal of Physiology·Y SugawaraH Sakamoto
Jan 1, 1993·Biological & Pharmaceutical Bulletin·M MochizukiS Hizatate
May 21, 1996·Biochemistry·C BalagopalakrishnaJ M Rifkind
Jan 1, 1995·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·M C MardenC Poyart
Apr 1, 1996·Protein Science : a Publication of the Protein Society·L R ManningJ M Manning
Jan 4, 1997·Biochimica Et Biophysica Acta·M Tsuruga, K Shikama

❮ Previous
Next ❯

Citations

Sep 19, 2006·Journal of the American Society for Mass Spectrometry·Brian L Boys, Lars Konermann
Dec 23, 2008·Journal of the American Society for Mass Spectrometry·P John Wright, D J Douglas
May 2, 2002·Biochimica Et Biophysica Acta·Jack A Kornblatt, M Judith Kornblatt
Jan 29, 2011·Nature·John S O'Neill, Akhilesh B Reddy
Apr 12, 2012·The Journal of Biological Chemistry·Jesús TejeroMark T Gladwin
May 22, 2002·Protein Science : a Publication of the Protein Society·Michael C MardenDonald J Abraham
Dec 10, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Maram T BashaPaul V Bernhardt
Sep 26, 2013·Antioxidants & Redox Signaling·John S O'Neill, Kevin A Feeney
Mar 28, 2008·Annals of Hematology·Emmanuel BisséHeinrich Wieland
Nov 26, 2015·Journal of Food Science·He CaiMark P Richards
Oct 22, 2010·Journal of Mass Spectrometry : JMS·Xian WangJianfeng Liu
Sep 27, 2005·Biophysical Chemistry·Shibsekhar RoyAnjan Kr Dasgupta
Dec 22, 2005·The FEBS Journal·Corinne Vasseur-GodbillonVéronique Baudin-Creuza
Aug 28, 1999·The Journal of Biological Chemistry·V Baudin-CreuzaJ Pagnier
Apr 18, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Carlos J MunozPedro Cabrales
Jul 15, 2020·Molecular Aspects of Medicine·Alessandra di MasiPaolo Ascenzi
Jun 22, 2021·International Journal of Biological Macromolecules·Selvaraju KanagarajanLi-Hua Zhu
Jan 24, 2002·Journal of Agricultural and Food Chemistry·Mark P Richards, Herbert O Hultin
Jul 9, 2004·Journal of Agricultural and Food Chemistry·Ingrid UndelandHerbert O Hultin
Mar 31, 2007·Journal of Agricultural and Food Chemistry·Mark P RichardsAlexandra C M Oliveira
Oct 13, 2006·Journal of Agricultural and Food Chemistry·Eric W Grunwald, Mark P Richards
Oct 31, 2007·Journal of Agricultural and Food Chemistry·Patroklos Vareltzis, Herbert O Hultin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Current Protein & Peptide Science
Gayathri Vasudevan, Melisenda J McDonald
Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
E IlkowskaG Stochel
© 2022 Meta ULC. All rights reserved